PAMAM树状大分子与5-氟尿嘧啶相互作用的NMR研究

doi:10.11938/cjmr20192743

波谱学杂志 ›› 2019, Vol. 36 ›› Issue (4): 555-562.doi: 10.11938/cjmr20192743

PAMAM树状大分子与5-氟尿嘧啶相互作用的NMR研究

宁彩芳^1,2, 马敏珺^1,2, 郭朝晖^1,2, 张书怀^1,2, 乔岩^1,2, 王英雄^1,2

1. 中国科学院山西煤炭化学研究所, 山西太原 030001;
2. 中国科学院大学材料与光电研究中心, 北京 100049

收稿日期:2019-04-26 出版日期:2019-12-05 发布日期:2019-05-30
通讯作者: 乔岩, 王英雄 E-mail:qiaoy@sxicc.ac.cn;wangyx@sxicc.ac.cn
基金资助:
国家自然科学基金资助项目（U1710106，U1810111）.

Interactions Between 5-Fluorouracil and PAMAM Dendrimers Studies by NMR Spectroscopy

NING Cai-fang^1,2, MA Min-jun^1,2, GUO Zhao-hui^1,2, ZHANG Shu-huai^1,2, QIAO Yan^1,2, WANG Ying-xiong^1,2

1. Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China;
2. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2019-04-26 Online:2019-12-05 Published:2019-05-30

1. 补充材料.pdf(148KB)

摘要/Abstract

摘要： 本文利用多种核磁共振（NMR）技术研究了聚酰胺-胺树状大分子（PAMAM）与5-氟尿嘧啶（5-FU）在水溶液中的相互作用.¹H NMR化学位移滴定结果表明5-FU分子结合在PAMAM分子表面，¹³C NMR化学位移滴定分析结果表明5-FU分子通过氢键或疏水间相互作用进入到PAMAM的内部疏水空腔.¹H核和¹⁹F核自旋-晶格弛豫时间（T₁）的测定结果也说明了二者之间存在相互作用.NOESY实验进一步验证了5-FU分子进入到PAMAM分子的内部空腔.本研究为研究树状大分子和药物小分子的相互作用提供了系统的NMR分析方法.

关键词: 液体核磁共振, 相互作用, 树状大分子PAMAM, 5-氟尿嘧啶

Abstract: Interactions between 5-fluorouracil (5-FU) and PAMAM in D₂O solution were studied by NMR spectroscopy. The results of ¹H NMR chemical shift titration experiments demonstrated that 5-FU molecules bonded to the surface of PAMAM, and the results of ¹³C NMR chemical shift titrations experiments disclosed that hydrogen-bond interactions and/or hydrophobic interaction ins existed in the interior pockets of PAMAM and 5-FU. These results were further supported by ¹H and ¹⁹F relaxation time measurements. The NOESY experiment was used to confirm the presence of 5-FU encapsulation in the PAMAM-5-FU complexes. This study provides a methodology for studying the interactions between dendrimers and drug molecules.

Key words: liquid-state NMR, interaction, PAMAM dendrimers, 5-fluorouracil

中图分类号:

O482.53

宁彩芳, 马敏珺, 郭朝晖, 张书怀, 乔岩, 王英雄. PAMAM树状大分子与5-氟尿嘧啶相互作用的NMR研究[J]. 波谱学杂志, 2019, 36(4): 555-562.

NING Cai-fang, MA Min-jun, GUO Zhao-hui, ZHANG Shu-huai, QIAO Yan, WANG Ying-xiong. Interactions Between 5-Fluorouracil and PAMAM Dendrimers Studies by NMR Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2019, 36(4): 555-562.

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PAMAM树状大分子与5-氟尿嘧啶相互作用的NMR研究

Interactions Between 5-Fluorouracil and PAMAM Dendrimers Studies by NMR Spectroscopy

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