Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by 1H NMR

Abstract

Abstract:

The chemical fingerprints of 27 Angelica sinensis samples harvested at different growth stages were analyzed by ¹H NMR. Ferulic acid, ligustilide and angelica polysaccharide, three of the active constituents of Angelica sinensis, were identified, with their ¹H chemical shifts assigned. The relative peak areas of characteristic peaks were used to descript the changes of chemical contents in Angelica sinensis at different growth stages. The results showed that the levels of three constituents had little changes during the first year. More changes were observed in year two and year three. During the growth cycle of Angelica sinensis, the changes of angelica polysaccharide were greater than those of the other two constituents, which were proven to be the active ingredients involved in metabolism. It is concluded that ¹H NMR fingerprinting and principle component analysis can be used to analyze the active ingredient of Angelica sinensis at different growth periods.

Key words: ¹H NMR, chemical fingerprinting, Angelica sinensis, growth stage, principal component analysis

CLC Number:

O482.53

YANG Ying-wen, WANG Ya-li*, SA Ri-na, PAN Xin-bo, XIAO Yu-qi, SUN Yu-jing, WANG Lan-xia. Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by ¹H NMR[J]. Chinese Journal of Magnetic Resonance.

References

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Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by ¹H NMR

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