Abstract:

Acarbose, an α - glucosidase inhibitor, has been widely used in the treatment of type-II diabetes due to its unique mechanism of action. Acarbose’s comprehensive and accurate ¹H-NMR and ¹³C-NMR data，as well as ¹H-NMR data of all hydroxyl groups of acarbose and ¹H-NMR and ¹³C-NMR data of the dual signal of C-ring α and β, were reported in DMSO-d₆ for the first time. The attribution of ¹H NMR and ¹³C NMR data were carried out by using DEPT-135, ¹H-¹H COSY, ¹H-¹³C HSQC, and ¹H-¹³C HMBC techniques. The molecular structure of Acarbose was confirmed, which provided a reliable data foundation for the safety and quality control of Acarbose drugs.

Key words: liquid-state NMR, Structural assignment,, 2D NMR, Acarbose