Chinese Journal of Magnetic Resonance

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NMR Analysis of Nicotinamide N-Oxide and Pseudouridine in Rat Urine

AN Yan-peng1,2,YANG Xiao-yan1,2,LI Hong-de1,2,LI Ning1,2,TANG Hui-ru1*


 
  

  1. 1. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-05-20 Revised:2013-07-21 Online:2014-06-05 Published:2014-06-05
  • About author:*Corresponding author:TANG Hui-ru, Tel: 027-87198430,E-mail:huiru.tang@wipm.ac.cn.
  • Supported by:

    国家自然科学基金资助项目(21175149, 21221064);国家重点基础研究发展计划资助项目(2010CB912501, 2012CB934004).

Abstract:

Nicotinamide N-oxide and pseudouridine are two important metabolites in rat urine. In this studies, the 1H and 13C NMR signals from these two metabolites were assigned using a series of two-dimensional (2D) NMR spectra including 1H-1H COSY, 1H-1H TOCSY, 1H-1H J-RES, 1H-13C HSQC and 1H-13C HMBC.

Key words: pseudouridine, liquid NMR, assignment, 2D NMR, nicotinamide N-oxide

CLC Number: