Metabolomic Analysis of Clostridium thermocellum

Chinese Journal of Magnetic Resonance

Previous Articles Next Articles

Metabolomic Analysis of Clostridium thermocellum

ZHU Xin-shu^1,2，CUI Jia-tao^1,2，FENG Yin-gang¹，ZHANG Jing-tao^1*，CUI Qiu^1,3*

1. Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China

Received:2013-05-30 Revised:2013-11-25 Online:2014-06-05 Published:2014-06-05
About author:*Corresponding author：ZHANG Jing-tao, Tel: 0532-80662705，E-mail：zhang_jt@qibebt.ac.cn；CUI Qiu, Tel: 0532-80662706，E-mail：cuiqiu@qibebt.ac.cn.
Supported by:
国家重点基础研究发展计划资助项目(2011CB7074040)，中国科学院科研装备研制项目(YZ201138)，国家高技术研究发展计划资助项目(2011BAD22B02)．

Abstract

Abstract:

Clostridium thermocellum is a thermophilic anaerobic gram-positive bacteria that can efficiently utilize lignocelluloses. It is one of the most important candidate microorganisms for cellulosic ethanol production with consolidated bioprocessing. Metabolome reflects the interaction between cell and its environmental factors, and is an important component of system biology. In this work, ¹H NMR and 2D NMR techniques were used to analysis the central metabolites of C. thermocellum. Some biologically significant metabolites were identified in the intracellular compartment of C. thermocellum, including such as cellodextrin, phosphoenolpyruvate and d-erythrose-4- phosphate. Preliminary multivariate statistical analysis of the wild type strain and ethanol tolerant strain showed various metabolic differences in several metabolic pathways including actively conversion of cellubiose to cellodextrin, enhancement of non-oxidative pentose phosphate pathway and suppression of Embden-Meyerhof-Parnas pathway.

Key words: liquid NMR, assignment, 2D NMR, Clostridium thermocellum, cellodextrin, metabolomics/metabonomics

CLC Number:

O482.53

ZHU Xin-shu1,2，CUI Jia-tao1,2，FENG Yin-gang1，ZHANG Jing-tao1*，CUI Qiu1,3* . Metabolomic Analysis of Clostridium thermocellum[J]. Chinese Journal of Magnetic Resonance.

References

[1] Lynd L R, van Zyl W H, McBride J E, et al. Consolidated bioprocessing of cellulosic biomass: an update[J]. Curr Opin Biotech, 2005, 16(5): 577－583.

[2] Brown S D, Guss A M, Karpinets T V, et al. Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum[J]. P Natl Acad Sci USA, 2011, 108(33): 13 752－13 757.

[3] Szeto S S W, Reinke S N, Sykes B D, et al. Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through H-1 NMR-based metabolic footprinting [J]. J Proteome Res, 2010, 9(12): 6 729－6 739.

[4] Zhao Xiu-ju(赵秀举), Wang Yu-lan(王玉兰). Applications of NMR based metabonomic approaches in the assessment of drug toxicity(代谢组NMR分析与药物毒理研究)[J]. Chinese J Magn Reson(波谱学杂志), 2011, 28(1): 1－17.

[5] Ye Y F, Zhang L M, Hao F H, et al. Global metabolomic responses of Escherichia coli to heat stress[J]. J Proteome Res, 2012, 11(4): 2 559－2 566.

[6] Yang S, Giannone R, Dice L, et al. Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress[J]. BMC Genomics, 2012, 13(1): 336－353.

[7] Yan Xian-zhong(颜贤忠), Zhao Jian-yu(赵剑宇), Peng Shuang-qing(彭双清), et al. Metabonomics in post-genomic era代谢组学在后基因组时代的作用)[J]. Chinese J Magn Reson(波谱学杂志), 2004, 21(2): 263－271.(

[8] Lindon J C, Holmes E, Nicholson J K. Recent developments and applications of NMR-based metabonomics[J]. Chinese J Magn Reson, 2006, 23(1): 101－127.

[9] Tian Yuan(田园), Tang Hui-ru(唐惠儒). Identification and structural determination of saccharides in rat feces(大鼠粪样中几种糖类物质的结构确定)[J]. Chinese J Magn Reson(波谱学杂志), 2012, 29(3): 361－371.

[10] Fan T W M, Lane A N. Structure-based profiling of metabolites and isotopomers by NMR[J]. Prog Nucl Magn Reson Spectrosc, 2008, 52(2-3): 69－117.

[11] Fan W M T. Metabolite profiling by one-and two-dimensional NMR analysis of complex mixtures[J]. Prog Nucl Magn Reson Spectrosc, 1996, 28(2): 161－219.

[12] Cui Q, Lewis I A, Hegeman A D, et al. Metabolite identification via the Madison Metabolomics Consortium Database[J]. Nat Biotechnol, 2008, 26 (2): 162－164.

[13] Matulova M, Delort A M, Nouaille R, et al. Concurrent maltodextrin and cellodextrin synthesis by Fibrobacter succinogenes S85 as identified by 2D NMR spectroscopy[J]. Eur J Biochem, 2001, 268(14): 3 907－3 915.

[14] Rydzak T, McQueen P D, Krokhin O V, et al. Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression[J]. BMC Microbiol, 2012, 12(1): 214－232.

[15] Herrero A A, Gomez R F, Roberts M F. ³¹P NMR-studies of Clostridium thermocellum-Mechanism of end product inhibition by ethanol[J]. J Biol Chem, 1985, 260(12): 7 442－7 451.

[16] Zhang Y H P, Lynd L R. Cellodextrin preparation by mixed-acid hydrolysis and chromatographic separation[J]. Anal Biochem, 2003, 322(2): 225－232.

Metabolomic Analysis of Clostridium thermocellum

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YIN Tian-peng, WANG Ya-rong, WANG Min, SHI Wen-zhi, ZHANG Zheng-qian, HE Sha-sha. Complete Assignments of NMR Spectral Data of Three C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 331-340.
[2]	WANG Ya-lan, WANG Xiao-jing, WANG Zhi-wei. Spectral Analyses and Structural Elucidation of Azilsartan [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 350-358.
[3]	LIU Ji-hong, JIN Kun, WANG Ping, LUO Gen. An NMR Study on Esculetin and It's Derivatives [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 341-349.
[4]	WEI Hui-qiang, YU Jiang, BI Chang-fen, NING Hong-xin, LI Yi-liang, LIU Qiang. An NMR Study of N-isobutyryl-3'-O-(1-fluoro-1,1,3,3-tetraisopropyl-1,3-disiloxane-3-yl)-2'-benzyloxycarbonyl-guanosine [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 93-102.
[5]	LI Ying-jun, YANG Kai-dong, JIN Kun, LIU Ji-hong, WANG Si-yuan, ZHANG Nan. NMR Study of A Novel Carbazole-Isatin Based Bis-Thiocarbohydrozone Derivative [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 83-92.
[6]	YU Gang-jin, ZHOU Zhi-ming, LV Ming, QIAN Sheng-tao, KONG Yu-hua, ZHANG Xu, MAO Shi-zhen, LIU Mai-li. Qualitative and Quantitative NMR Analysis of 1,2-Butanediol in Polymer Grade Coal-Based Ethylene Glycol [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 55-64.
[7]	ZHANG Ming-guang, FENG Xiao-hu, YU Shui-tao, JI Xiao-long, CHEN Zai-xin. Spectral Analysis and Structural Elucidation of Besifloxacin Hydrochloride [J]. Chinese Journal of Magnetic Resonance, 2018, 35(3): 374-384.
[8]	ZHANG Shi-mao, ZHANG Shao-nan, GE Xiang, WU Jian-meng. Optimal Design and Application of Two-Dimensional NMR Logging in Chuanxi Tight Gas Reservoir [J]. Chinese Journal of Magnetic Resonance, 2018, 35(2): 234-242.
[9]	ZHOU Zhong-gao, YUAN Yang-yang, HUANG Li, LIU Jin-xiang, XIE Yong-rong. Spectral Analysis of Glucose-Based Chiral N-heterocyclic Carbene Precursors [J]. Chinese Journal of Magnetic Resonance, 2018, 35(2): 215-225.
[10]	CHAI Xin, SUN Peng, YUAN Bin, XIAO Xiong-jie, ZHANG Xu, LIU Mai-li. Fast Detection of Choline Containing Compounds in Dairy Products Using NMR [J]. Chinese Journal of Magnetic Resonance, 2018, 35(2): 178-187.
[11]	YIN Tian-peng, CHEN Yang, LUO Ping, CAL Le, DING Zhong-tao. Structural Elucidation and NMR Spectral Assignments of Two C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2018, 35(1): 90-97.
[12]	ZHOU Zhong-gao, YUAN Yang-yang, LIU Hong-bo, QI Qi, XIE Yong-rong. An NMR Study on Prucalopride [J]. Chinese Journal of Magnetic Resonance, 2018, 35(1): 119-127.
[13]	LIU Fu-fa, YANG Xiao-yan, HAO Fu-hua, WANG Yu-lan, TANG Hui-ru. Assignments of ¹H and ¹³C NMR Signals of 26 Metabolites Associated with the Shikimate Pathway [J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 311-322.
[14]	SUN Wei, SHE Meng-yao, ZONG Chun-lei, GAO Xiang, GUO Juan. An NMR Study on Diacetonefructose [J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 329-337.
[15]	ZHANG En, CUI De-yun, SUN Wei, LI Peng-yun, XU Jin-mei, LIU Hong-min. Structural Elucidation of a Hydrolysis Product from Derivatives of Allofuranose [J]. Chinese Journal of Magnetic Resonance, 2017, 34(2): 183-190.