Fast Detection of Choline Containing Compounds in Dairy Products Using NMR

doi:10.11938/cjmr20172581

Abstract

Abstract: Choline containing compounds (CCCs) are a class of small molecule metabolites with important biological functions. Some CCCs can be synthesized by human body, but most of them come from daily diet. CCCs exist in milk, beans, and infant formulas additives. Chronic choline deficiency in the diet is associated with diseases such as organ dysfunction. Therefore, fast detection for CCCs is important for food research. Nuclear magnetic resonance (NMR) spectroscopy is a noninvasive method for CCCs measurement. In this study, ¹H-¹⁴N SOFAST-HMQC was used to measure the CCCs in dairy products. It was observed that CCCs detection by this method can be more selective than traditional one-dimensional ¹H NMR, and the detection sensitivity per unit time was 1.5~2.0 times higher than that of the previous developed ¹H-¹⁴N HSQC method.

Key words: liquid NMR, ¹H-¹⁴N SOFAST-HMQC, choline, dairy products

CLC Number:

O482.53

CHAI Xin, SUN Peng, YUAN Bin, XIAO Xiong-jie, ZHANG Xu, LIU Mai-li. Fast Detection of Choline Containing Compounds in Dairy Products Using NMR[J]. Chinese Journal of Magnetic Resonance, 2018, 35(2): 178-187.

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