Metabolic Changes in Prefrontal Cortex and Hippocampus of Rats with Generalized Anxiety Revealed by In Vivo 1H-MRS

Abstract

Abstract:

Metabolic changes in bilateral prefrontal cortex and hippocampus of rats with generalized anxiety disorder were investigated by ¹H magnetic resonance spectroscopy (¹H-MRS) performed at 7.0 T. Male Wistar rats were randomized assigned into the control and model groups (n =8 each). Chronic emotional stress-induced anxiety was used as the generalized anxiety disorder model. Behavioral abnormalities in the model rats were assessed with the elevated plus-maze test. Levels of cerebral metabolites, including N-acetyl aspartate (NAA), choline (Cho), creatine (Cr) and glutamate (Glu), in bilateral prefrontal cortex and hippocampus were measured and used to calculate NAA/Cr, Cho/Cr and Glu/Cr ratios. No significant metabolic difference were found between the model rats and the control rats in bilateral hippocampus and left prefrontal cortex. However, the model rats were found to have significantly lower NAA/Cr and Cho/Cr and significantly increased Glu/Cr in right prefrontal cortex, relative to the control rat. It is concluded that chronic anxiety may be associated with imbalance of amino acid neurotransmitters, neuronal injury/loss and intracellular signal transduction abnormalities in the right prefrontal cortex.

Key words: NMR, rat, generalized anxiety disorder, ¹H magnetic resonance spectroscopy, prefrontal cortex, hippocampus

CLC Number:

O482.53
Q591

LI Ning, TANG Qi-Sheng, HOU Xiu-Juan, ZHAO Rui-Zhen, HUANG Yu-Ling, FANG Fang, ZHANG Feng-Chao . Metabolic Changes in Prefrontal Cortex and Hippocampus of Rats with Generalized Anxiety Revealed by In Vivo ¹H-MRS[J]. Chinese Journal of Magnetic Resonance, 2010, 27(2): 185-193.

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Metabolic Changes in Prefrontal Cortex and Hippocampus of Rats with Generalized Anxiety Revealed by In Vivo ¹H-MRS

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