Improvement of Fluorescence Quantum Yield for Terbium (III)-Bound Lanthanide-Binding Tag

doi:10.11938/cjmr20160110

Abstract

Abstract:

Long-lived luminescence lifetime and large Stokes shift of the lanthanide ion made it widely used in fluorescence detection. The lanthanide-binding tag (LBT) is a small, genetically encoded, versatile protein fusion partner that specifically binds a lanthanide ion with high affinity (K_D ≈10^-9mol/L). These properties make LBT wildly used for studies on protein structures and functions. Lanthanide in LBT cannot be excited directly, and tryptophan is needed to serves as a sensitizer to absorb energy and transfer energy to the lanthanide. We introduced LBT onto the C-terminal of ubiquitin (Ub), and increased the number of indole rings in LBT using site-directed mutagenesis to increase the quantum yield of Tb (III)-bound Ub-LBT.

Key words: fluorescence spectroscopy, lanthanide-binding tag (LBT), quantum yield, dissociation constant, ubiquitin (Ub)

CLC Number:

O482.53

CHEN Shao-min, TANG Chun, GONG Zhou. Improvement of Fluorescence Quantum Yield for Terbium (III)-Bound Lanthanide-Binding Tag[J]. Chinese Journal of Magnetic Resonance, 2016, 33(1): 106-116.

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