Evaluation of Separation Performance of Polydimethylsiloxane-Matrixed Chromatography by NMR

doi:10.11938/cjmr20150312

Chinese Journal of Magnetic Resonance ›› 2015, Vol. 32 ›› Issue (3): 511-517.doi: 10.11938/cjmr20150312

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Evaluation of Separation Performance of Polydimethylsiloxane-Matrixed Chromatography by NMR

WU Rui¹，BAI Zheng-wu^1*，YANG Ying²，HUANG Shao-hua^2*

1. School of Chemical and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, China; 2. Key Laboratory of Biobased-materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China

Received:2014-07-15 Revised:2015-07-20 Online:2015-09-05 Published:2015-09-05
About author:*Corresponding author: BAI Zheng-wu, Tel: +86-027-87194980, E-mail: zhengwu_bai@yahoo.com; HUANG Shao-hua, Tel: +86-0532-80662660, E-mail: huangsh@qibebt.ac.cn.
Supported by:
国家自然科学基金资助项目(21105108)

Abstract

Abstract:

Polydimethylsiloxane (PDMS) has been used as the virtual “stationary phase” (VSP) for chromatographic NMR. Its separation capability towards halogenated compounds was investigated. Diffusion coefficients of n-pentane, 1-bromopentane and 1,5-dibromopentane contained in a mixture were measured with high-resolution liquid-state diffusion-ordered NMR spectroscopy (DOSY). In the absence of PDMS, these diffusion coefficients were close, and as a result, the three components could not be separated. In contrast, the diffusion coefficients were markedly different from each other in the presence of PDMS, leading to excellent separation. In addition, the effects of sample temperature and solution viscosity on separation performance were also investigated. It was observed that separation performance improved as sample temperature rose; and reduced as solution viscosity increased.

Key words: polydimethylsiloxane, virtual stationary phase, chromatography NMR, DOSY, separation

CLC Number:

O482.53

WU Rui1，BAI Zheng-wu1*，YANG Ying2，HUANG Shao-hua2*. Evaluation of Separation Performance of Polydimethylsiloxane-Matrixed Chromatography by NMR[J]. Chinese Journal of Magnetic Resonance, 2015, 32(3): 511-517.

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Evaluation of Separation Performance of Polydimethylsiloxane-Matrixed Chromatography by NMR

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