大鼠中风模型的超小氧化铁粒子神经血管成像

波谱学杂志

大鼠中风模型的超小氧化铁粒子神经血管成像

沈伊民1*，郑伟丽1，CHENG Yu-Chung N1，丁玉川2，HIGASHIDA Tetsuhiro2，李杰2，叶永泉1，RAYNAUD Jean-Sebastien3，HAACKE E Mark1,4

1. 美国韦恩州立大学医学院放射系，底特律 48201，美国；
2. 美国韦恩州立大学医学院神经外科系，底特律 48201，美国；
3. 法国格尔伯实验室, 欧奈苏布瓦93600, 法国；
4. 美国韦恩州立大学生物医学工程系，底特律 48201，美国

收稿日期:2013-10-16 修回日期:2013-11-12 出版日期:2014-03-05 发布日期:2014-03-05
作者简介:SHEN Yi-min(1961-), male, born in Jiangsu, PhD., his research focuses on MRI, Tel.: +01-313-966-0579, fax:+01-313-745-9182. E-mail: ym_shen@wayne.edu. *通讯联系人：沈伊民.
基金资助:
Heart and Stroke Foundation of Canada (HSFC), Synchrotron Medical Imaging Team Grant (# CIF 99472).

USPIO High Resolution Neurovascular Imaging in a Rat Stroke Model of Transient Middle Cerebral Artery Occlusion

SHEN Yi-min1*，ZHENG Wei-li1，CHENG Yu-chung N1，DING Yu-chuan2，HIGASHIDA Tetsuhiro2，LI Jie2，YE Yong-quan1，RAYNAUD Jean-Sebastien3，HAACKE E Mark1,4

1. Department of Radiology, Wayne State University School of Medicine, Detroit 48201, USA;
2. Department of Neurological Surgery, Wayne State University School of Medicine, Detroit 48201, USA;
3. Guerbet Research, Aulnay-Sous-Bois 93600, France;
4. Department of Biomedical Engineering, Wayne State University, Detroit 48201, USA

Received:2013-10-16 Revised:2013-11-12 Online:2014-03-05 Published:2014-03-05
About author:SHEN Yi-min(1961-), male, born in Jiangsu, PhD., his research focuses on MRI, Tel.: +01-313-966-0579, fax:+01-313-745-9182. E-mail: ym_shen@wayne.edu. *Corresponding author
Supported by:
Heart and Stroke Foundation of Canada (HSFC), Synchrotron Medical Imaging Team Grant (# CIF 99472).

摘要/Abstract

摘要：

现今诱导血管增生剂在中风后的治疗效应引起了人们的关注．这项工作的一个目的是用短时脑中动脉栓塞大鼠中风模型(MCAO)和磁化率加权成像(SWI)的核磁共振成像(MRI)方法，监测在中风后半月形损伤区新生成的旁侧血管．P904 是法国格尔伯实验室生产的超小超顺磁氧化铁粒子弛豫试剂(USPIO)．它在低剂量减少T₁ 弛豫时间，适中剂量时减少T₂*弛豫时间．实验动物被随机分为3 组：中风Sildenafil 治疗组(n=6)、中风无治疗对照组(n=5)和无中风无治疗对照组(n=1)．在P904 注入前后分别进行MRI成像．磁化率加权成像的时间点是：栓塞手术前、栓塞手术后24 小时、栓塞手术后两周和四周．结果表明，术后两周，在治疗组中中风严重的动物的缺血区的周边显示了MRI 可见的新生血管．结论：在短时脑中动脉栓塞大鼠中风模型中，使用超小超顺磁氧化铁粒子弛豫试剂和7 T 高分辨磁化率加权成像能够监测半月形损伤区新生血管的形成．

关键词: 核磁共振成像(MRI) 中风血管增生, 磁化率加权成像, 超小超顺磁氧化铁粒子, 神经血管

Abstract:

Angiogenesis inducing agents, such as Sildenafil, can be used to treat stroke. In this study, USPIO-enhanced MRI was used to visualize collateral vessels in the penumbral area of lesions of rats subjected to transient middle cerebral artery occlusion (tMCAO), and the evaluate efficacy of the Sildenafil treatment. Here, P904, a USPIO agent, was used as a T₁ reducing agent at a low dose for MR angiography and as a T₂* reducing agent at a moderate dose for
visualization of very small vessels on gradient echo susceptibility-weighted imaging (SWI). SWI scans without/with USPIO injection were performed for the following cases: tMCAO without any treatment (n=5), tMCAO with Sildenafil treatment (n=6), and sham tMCAO (n=1) at four different time points (i.e., baseline, 24 h, two weeks and four weeks after tMCAO). The presence of newly developed vessels in the periphery of the ischemic core was only observed in those
animals showing severe ischemic brain damage and at the same time received Sildenafil treatment at two weeks post tMCAO. This work suggests that, at 7 T, high resolution SWI in conjunction with USPIO injection at a moderate dose can be used to visualize post-stroke angiogenesis in the tMCAO rat model.

Key words: MRI, stroke, angiogenesis, SWI, USPIO, neurovasculature

中图分类号:

O482.53

沈伊民1*，郑伟丽1，CHENG Yu-Chung N1，et al.. 大鼠中风模型的超小氧化铁粒子神经血管成像[J]. 波谱学杂志.

SHEN Yi-min1*，ZHENG Wei-li1，CHENG Yu-chung N1，DING Yu-chuan2，HIGASHIDA Tetsuhiro2，LI Jie2，YE Yong-quan1，RAYNAUD Jean-Sebastien3，HAACKE E Mark1,4.

USPIO High Resolution Neurovascular Imaging in a Rat Stroke Model of Transient Middle Cerebral Artery Occlusion

[J]. Chinese Journal of Magnetic Resonance.

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