9.4 T下TX模型小鼠脑组织的扩散张量成像研究

doi:10.11938/cjmr20192749

波谱学杂志 ›› 2019, Vol. 36 ›› Issue (4): 510-516.doi: 10.11938/cjmr20192749

9.4 T下TX模型小鼠脑组织的扩散张量成像研究

鲁晨^1,2, 董健健^1,2, 钟凯¹

1. 中国科学院强磁场科学中心, 安徽合肥 230031;
2. 中国科学技术大学, 安徽合肥 230026

收稿日期:2019-05-15 出版日期:2019-12-05 发布日期:2019-08-20
通讯作者: 钟凯 E-mail:kzhong@hmfl.ac.cn
基金资助:
国家自然科学基金重大研究计划（91649101）；中科院合肥大科学中心重点研发项目（2017HSC-KPRD003）.

Diffusion Tensor Imaging on TX Mice Brain at 9.4 T

LU Chen^1,2, DONG Jian-jian^1,2, ZHONG Kai¹

1. High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China;
2. University of Science and Technology of China, Hefei 230026, China

Received:2019-05-15 Online:2019-12-05 Published:2019-08-20

摘要/Abstract

摘要： 本文首次应用9.4 T高场磁共振扩散张量成像（diffusion tensor imaging，DTI）技术，研究了Wilson疾病模型TX（toxic milk）小鼠的脑组织微观结构改变和结构连接情况.基于感兴趣区域（region of interest，ROI）的分析发现，与对照组相比，TX模型组的各向异性比值（fractional anisotropy，FA）在海马、尾状核和苍白球显著下降；平均扩散率（mean diffusivity，MD）则呈现上升趋势，但无统计学意义.纤维束追踪法结果表明TX模型组小鼠的脑结构连接并未受到严重破坏，证明了铜累积对脑组织的损伤具有区域性.

关键词: 9.4 T, 扩散张量成像(DTI), TX模型小鼠, 各向异性比值, 平均扩散率

Abstract: Diffusion tensor imaging (DTI) was used to evaluate micro-structural changes and structural connectivity in the TX mice model of Wilson's disease (WD) at 9.4 T. Region of interest (ROI) analysis showed that mean fractional anisotropy (FA) values in the hippocampus, caudate putamen and lateral globus pallidus of the TX mice decreased significantly, relative to those in the control mice. The mean diffusivity (MD) values in these regions showed no significant inter-group differences. The results of fiber tracking demonstrated that structural connectivity of in the brain of TX mice was maintained, indicating that the effects of copper accumulation in these mice were mainly regional.

Key words: 9.4 T, diffusion tensor imaging (DTI), TX mice, fractional anisotropy, mean diffusivity

中图分类号:

鲁晨, 董健健, 钟凯. 9.4 T下TX模型小鼠脑组织的扩散张量成像研究[J]. 波谱学杂志, 2019, 36(4): 510-516.

LU Chen, DONG Jian-jian, ZHONG Kai. Diffusion Tensor Imaging on TX Mice Brain at 9.4 T[J]. Chinese Journal of Magnetic Resonance, 2019, 36(4): 510-516.

参考文献

[1] BULL P C, THOMAS G R, ROMMENS J M, et al. The Wilson disease gene is a putative copper transporting P-type ATPase similar to the Menkes gene[J]. Nat Genet, 1993, 5(4):327-337.
[2] DAVIES K M, HARE D J, COTTAM V, et al. Localization of copper and copper transporters in the human brain[J]. Metallomics, 2013, 5(1):43-51.
[3] PATIL M, SHETH K A, KRISHNAMURTHY A C, et al. A review and current perspective on Wilson disease[J]. J Clin Exp Hepatol, 2013, 3(4):321-336.
[4] ZHAO W, CHENG N, HAN Y Z. Advances in animal models of Wilson's disease[J]. Anhui Medical Journal, 2014, 35(11):1611-1613. 赵雯, 程楠, 韩咏竹. Wilson病的动物模型研究进展[J]. 安徽医学, 2014, 35(11):1611-1613.
[5] FUENTEALBA I C, ABURTO E M. Animal models of copper-associated liver disease[J]. Comp Hepatol, 2003, 2(1):5.
[6] MEDICI V, HUSTER D. Animal models of Wilson disease[J]. Handb Clin Neurol, 2017, 142:57-70.
[7] TERWEL D, LÖSCHMANN Y N, SCHMIDT H H, et al. Neuroinflammatory and behavioural changes in the Atp7B mutant mouse model of Wilson's disease[J]. J Neurochem, 2011, 118(1):105-112.
[8] JIANG F, WANG Y J. Construction of human brain templates with diffusion tensor imaging data:a review[J]. Chinese J Magn Reson, 2018, 35(4):123-133. 蒋帆, 王远军. 扩散张量成像的人脑模板构建[J]. 波谱学杂志, 2018, 35(4):123-133.
[9] BUDDE M D, SKINNER N P. Diffusion MRI in acute nervous system injury[J]. J Magn Reson, 2018, 292:137-148.
[10] NIE T T, SHEN Z W, GENG K, et al. T2-weighted image and diffusion tensor imaging of cuprizone-induced demyelination in C57BL/6 mouse model[J]. Chinese J Magn Reson Imaging, 2014, 5(6):467-472. 聂婷婷, 沈智威, 耿宽, 等. Cuprizone诱导的C57BL/6小鼠脱髓鞘模型的T2WI及DTI研究[J]. 磁共振成像, 2014, 5(6):467-472.
[11] MASUTANI Y, AOKI S, ABE O, et al. MR diffusion tensor imaging:recent advance and new techniques for diffusion tensor visualization[J]. Eur J Radiol, 2003, 46(1):53-66.
[12] JADAV R, SAINI J, SANJIB S, et al. Diffusion tensor imaging (DTI) and its clinical correlates in drug native Wilson's disease[J]. Metab Brain Dis, 2013, 28(3):455-462.
[13] LI W, LIU J, SKIDMORE F, et al. White matter microstructure changes in the thalamus in Parkinson disease with depression:a diffusion tensor MR imaging study[J]. AJNR Am J Neuroradiol, 2010, 31(10):1861-1866.
[14] WANG A Q, WU H L, XU C S, et al. Study on lesion assessment of Cerebello-Thalamo-Cortical network in Wilson's disease with diffusion tensor imaging[J]. Neural Plast, 2017:7323121.
[15] MEENAKSHI-SUNDARAM S, MAHADEVAN A, TALY A B, et al. Wilson's disease:A clinico-neuropathological autopsy study[J]. J Clin Neurosci, 2008, 15(4):409-417.
[16] KONSMAN J P. The mouse brain in stereotaxic coordinates[M]//PAXINOS G, FRANKLIN K B J. Psychoneuroendocrinology. 2^nd Ed. New York:Academic Press, 2003, 28(6):827-828.
[17] BEHRENS T E, BERG H J, JBABDI S, et al. Probabilistic diffusion tractography with multiple fibre orientations:What can we gain?[J]. Neuroimage, 2007, 34(1):144-155.
[18] MEDICI V, HUSTER D. Animal models of Wilson disease[J]. Handb Clin Neurol, 2017, 142:57-70.
[19] PRZYBYLKOWSKI A, GROMADZKA G, WAWER A, et al. Neurochemical and behavioral characteristics of toxic milk mice:an animal model of Wilson's disease[J]. Neurochem Res, 2013, 38(10):2037-2045.
[20] GAO W J, LI Q, CHEN P Y, et al. The correlation between the structural properties of the arcuate bundle and the performance of language comprehension is analyzed by limiting spherical convolution[J]. Chinese J Magn Reson, 2016, 33(2):269-280. 高雯菁, 李锵, 陈品元, 等. 应用限制球形卷积解析弓状束的结构特性与语言理解表现的相关性[J]. 波谱学杂志, 2016, 33(2):269-280.
[21] LAWRENCE A, SAINI J, SINHA S, et al. Improvement of diffusion tensor imaging (DTI) parameters with decoppering treatment in Wilson's disease[J]. JIMD Rep, 2015, 25:31-37.
[22] HU J, JIAO X T, LIU X Q, et al. Cell apoptosis and expression of ATP7A in brain of Atp7b^tx-j mice[J]. Journal of Shanghai Jiaotong University (Medical Science). 2013, 33(7):916-919. 胡璟, 焦先婷, 刘晓青, 等. Atp7b^tx-j小鼠脑组织细胞凋亡及ATP7A表达研究[J]. 上海交通大学学报(医学版), 2013, 33(7):916-919.
[23] BONILLA E, SALAZAR E, VILLASMIL J J, et al. Copper distribution in the normal human brain[J]. Neurochem Res, 1984, 9(11):1543-1548.

9.4 T下TX模型小鼠脑组织的扩散张量成像研究

Diffusion Tensor Imaging on TX Mice Brain at 9.4 T

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价