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高分辨魔角旋转核磁共振技术(HR/MAS)在固相合成中的应用

贺文义1,姚念环1,Kit S. LAM2,刘刚1*   

  1. 1.中国医学科学院 中国协和医科大学药物研究所, 北京 100050;
    2.University of California, Davis Cancer Center, 4501 X Street, Sacramento, California 95817,USA
  • 收稿日期:2003-03-20 出版日期:2003-06-05 发布日期:2003-06-05
  • 作者简介:贺文义(1954-),男,北京人,副主任技师. *通讯联系人: 刘刚
  • 基金资助:

    “863”项目(2001AA234061, 2001AA234021-04, 2002AA2Z343B).

APPLICATIONS OF HIGH RESOLUTION MAGIC ANGLE SPINNING NMR TO SOLID PHASE SYNTHESIS

HE Wen-yi1, YAO Nian-huan1, KIT S. Lam2,LIU Gang1*   

  1. 1.Chinese Academy of Medical Sciences & Peking Union College of Medicine, Institute of Materia Medica, 1 Xian Nong Tan Street, Beijing 100050, China;
     2.University of California, Davis Cancer Center, 4501 X Street, Sacramento, California 95817, USA
  • Received:2003-03-20 Online:2003-06-05 Published:2003-06-05
  • About author:*Correspondence author:Liu Gang
  • Supported by:

    “863”项目(2001AA234061, 2001AA234021-04, 2002AA2Z343B).

摘要:

使用微量探头分析了不同树脂、溶剂、温度对1H NMR线宽的影响,并运用高分辨魔角旋转核磁共振技术跟踪固相载体上羟基氨基酸的糖基化反应. 该研究表明使用高分辨魔角旋转核磁共振探头可以快速、直接鉴定树脂上的产物,是固相合成中重要的分析工具.

关键词: 核磁共振,  高分辨魔角旋转核磁共振, 树脂, 固相合成

Abstract:

General conditions that affect High Resolution Magic Angle Spinning NMR (HR/MAS) were studied in this paper including five resins, five solvents and t
emperature. A Solid phase synthesis of o-glycosylated Fmoc protected amino acid was then monitored. The results demonstrated that both resin and the solvent are critical factors in affection of acquisition of NMR singlets.

Key words: NMR, high resolution magic angle spinning NMR (HR/MAS), resin, solid phase synth

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