昆明小鼠常见体液与组织代谢组NMR分析

波谱学杂志

昆明小鼠常见体液与组织代谢组NMR分析

蔡毅^1,2，豪富华¹，王玉兰^1*

1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心(中国科学院武汉物理与数学研究所)，湖北武汉 430071； 
2. 中国科学院大学，北京 100049

收稿日期:2012-05-15 修回日期:2012-06-08 出版日期:2013-03-05 发布日期:2013-03-05
通讯作者: 王玉兰，电话：027-87197143，E-mail：yulan.wang@wipm.ac.cn. E-mail:yulan.wang@wipm.ac.cn
作者简介:蔡毅（1985-），男，河北人，硕士研究生，主要从事啮齿动物的体液及组织代谢组的动态变化规律研究
基金资助:
国家重点基础研究发展计划(“973”计划)资助项目(2009CB118804).

NMR-Based Metabonomics Analysis of Biofluids and Tissues in Kunming Mice

CAI Yi^1,2，HAO Fu-hua¹，WANG Yu-lan^1*

1. State key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center of Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China; 
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-05-15 Revised:2012-06-08 Online:2013-03-05 Published:2013-03-05
Contact: Wang Yu-lan，Tel：027-87197143，E-mail：yulan.wang@wipm.ac.cn. E-mail:yulan.wang@wipm.ac.cn
About author:蔡毅（1985-），男，河北人，硕士研究生，主要从事啮齿动物的体液及组织代谢组的动态变化规律研究
Supported by:
国家重点基础研究发展计划(“973”计划)资助项目(2009CB118804).

摘要/Abstract

摘要：

昆明小鼠是我国特有且广泛使用的模式动物. 利用核磁共振(NMR)技术对昆明小鼠血液、尿液和肝脏等组织中的代谢物进行了较为系统的研究. 通过对昆明小鼠体液和组织中代表性的¹H NMR谱图，并结合COSY(Correlation Spectroscopy)，TOCSY(Total Correlation Spectroscopy)，J-Res(J-Resolved Spectroscopy)，HSQC(Heteronuclear Single Quantum Coherence)，HMBC(Heteronuclear Multiple Bond Coherence)等2D NMR谱图进行分析，共解析出82种小分子代谢物，其中尿液中约有46种，血样中约有32种，肝脏提取物中约有43种，肾脏皮质提取物中约有39种，部分代谢物在不同样品均存在. 这为进一步利用昆明小鼠为动物模型的研究提供了基础信息.

关键词: 核磁共振（NMR）, 昆明小鼠, 体液, 代谢组

Abstract:

Kunming (KM) mouse is a widely used model animal species in China. We measured the metabolites in plasma, urine, liver and other tissues from KM mice with nuclear magnetic resonance (NMR)-based metabonomic analysis. The representative ¹H NMR spectra of biofluids and tissues from KM mice were assigned with the aid of a range of two-dimensional NMR techniques, including COSY (Correlation Spectroscopy), TOCSY (Total Correlation Spectroscopy), J-Res (J-Resolved Spectroscopy), HSQC (Heteronuclear Single Quantum Coherence) and HMBC (Heteronuclear Multiple Bond Coherence). A total of 82 different metabolites, including 46 metabolites from the urine, 32 from the plasma, 43 from the liver extracts and 39 from the kidney cortex extracts, were identified. It was found that some metabolites existed in all the samples. Our results provide a foundation for future metabonomic studies using KM mice as the model animal.

Key words: NMR, Kunming mice, biofluids, metabonomics

中图分类号:

O482.53

蔡毅，豪富华，王玉兰*. 昆明小鼠常见体液与组织代谢组NMR分析[J]. 波谱学杂志.

CAI Yi，HAO Fu-hua，WANG Yu-lan*. NMR-Based Metabonomics Analysis of Biofluids and Tissues in Kunming Mice[J]. Chinese Journal of Magnetic Resonance.

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