波谱学杂志 ›› 1994, Vol. 11 ›› Issue (1): 77-84.

• 研究论文 • 上一篇    下一篇

小白鼠肌肉组织的NMR质子自旋交换分析

王立锦1, 贾明1, 刘太馨1, 靳键2   

  1. 1. 河北师范学院, 石家庄 050091;
    2. 南开大学, 天津 300071
  • 收稿日期:1992-09-18 修回日期:1993-06-29 出版日期:1994-03-05 发布日期:2018-01-20

NMR PROTON SPIN EXCHANGE ANALYSIS ON HEALTHY FRESH MOUSE MUSCLE TISSUES

Wang Lijin1, Jia Ming1, Liu Taixin1, Jin Jian2   

  1. 1. Hebei Teachers College, Shijiazhuang 050091;
    2. Nankai University, Tianjin 300071
  • Received:1992-09-18 Revised:1993-06-29 Online:1994-03-05 Published:2018-01-20

摘要: 本文在Zimmerman-Brittin两相质子交换核磁共振弛豫模型基础上,分析了NMR弛豫实验中检测信号与各相表现和本征弛豫多数的关系,编写了自动化处理实验数据的计算机程序,这一技术可用于复相系统中不同成分的NMR表现和本征弛豫特性研究中,本文中的样品是选用健康新鲜的小白鼠肌肉,没加任何处理,用h-h,s-h,s-s脉冲序列,反转恢复法(π-τ-π/2)在强场下(0.92T)做T1、T2测定实验,分析结果表明本征弛豫参数T1=1050ms,T2=4500μs的成分是由肌肉中的"自由水"引起的,其质子相对含量为69%;本征弛豫参数T1=530ms,T2=26μs的成分是由肌肉中的"束附水"引起的,其质子相对含量为9%,本征弛豫多数T1=530ms,T2=1250μs的成分是由肌肉中的各种大分子和有机物引起的,其质子相对含量为9%,本征弛豫参数T1=470ms,T2=1250μs的成分由样品中的脂肪引起的,其质子相对含量为13%,在肌肉组织中的质子与水中质子之间有强烈的交换作用,其交换率k=1000s-1.在脂肪中的质子与其它成分之间没有交换作用。

关键词: 核磁共振, 表观弛豫, 本征弛豫, 自旋交换

Abstract: Based on Zimmerman-Brittins model of spin exchange between two phases,the line-shape relaxation correlation technique, also called spin-grouping technique,was analysed.The programs processing experimental data are complied.This technique can be used in the study of NMR apparent and inherent relaxation characteristic of different components in heterogeneous system.In this paper the sample is the healthy fresh mouse muscle.T1 experment(π-τ-π/2) was performed using h-h,s-h,s-s pulse sequence at high field(0.9T).The results indicate that the T1=1050 ms,T2=4500μs component is caused by "free water" in muscle, the proton relative content of which is 69%;the T1=530 ms,T2=26 μs component is caused by "bound water"in muscle,the proton relative content of which is 9%, the T1=530 ms,T2=1250μs component is caused by various large molecules and organic compounds in muscle,the proton relative content is 9%;the T1=470 ms,T2=1250 μs componentis eaused by the fat in the sample,the proton relative content is 13%. Between theproton in muscle tissues and the proton in water fast exchange is dominant but thereis no exchange between the proton in fat and other components.

Key words: NMR, Apparent relaxation, Inherent relaxation, Spin exchange