三种氘标记的葡萄糖在大鼠脑胶质瘤细胞中的代谢特征对比

doi:10.11938/cjmr20233048

波谱学杂志 ›› 2023, Vol. 40 ›› Issue (3): 239-245.doi: 10.11938/cjmr20233048

三种氘标记的葡萄糖在大鼠脑胶质瘤细胞中的代谢特征对比

方羿^1,²,万倩²,袁家文²,林绍强¹,李烨²,刘新²,郑海荣²,邹超^2,^*()

1.广东药科大学，广东广州 510006
2.Paul C. Lauterbur生物医学成像研究中心，中国科学院深圳先进技术研究院，广东深圳 518055

收稿日期:2023-01-03 出版日期:2023-09-05 在线发表日期:2023-03-23
通讯作者: *Tel: 0755-86392248, E-mail: chao.zou@siat.ac.cn.
基金资助:
广东省自然科学基金资助项目(2022B1515120068);广东省自然科学基金资助项目(2020B1212060051);中国科学院全球共性挑战专项(321GJHZ2022081GC);国家重点研发计划资助项目(2022YFA1004203);国家重点研发计划资助项目(2021YFE0204400);国家重点研发计划资助项目(2021YFF0501500);中国科学院青促会基金(Y2021098);深圳市基础研究计划资助项目(JCY20200109110612375)

Comparison Study of the Metabolic Characteristics of Three Kinds of Deuterium-labeled Glucose in Rat Glioma Cells

FANG Yi^1,²,WAN Qian²,YUAN Jiawen²,LIN Shaoqiang¹,LI Ye²,LIU Xin²,ZHENG Hairong²,ZOU Chao^2,^*()

1. Guangdong Pharmaceutical University, Guangzhou 510006, China
2. Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

Received:2023-01-03 Published:2023-09-05 Online:2023-03-23
Contact: *Tel: 0755-86392248, E-mail: chao.zou@siat.ac.cn.

摘要/Abstract

摘要：

磁共振氘（²H）成像是近年来涌现出的一种能在活体水平下实现特定代谢通路可视化的分子代谢成像方法，在癌症检测等重大疾病方面具有极好的临床应用前景．本文分析了三种不同氘标记葡萄糖——[6,6’-²H₂]-葡萄糖、[2,3,4,6,6’-²H₅]-葡萄糖和[1,2,3,4,5,6,6’-²H₇]-葡萄糖在脑胶质瘤细胞中的代谢特征差异．将大鼠脑胶质瘤C6细胞与三种氘标记的葡萄糖一起孵育，收集不同时刻细胞培养基样品，采用核磁共振波谱仪对样品进行核磁共振氘谱采集，获取不同时间三种氘标记葡萄糖的消耗量以及下游代谢产物水和乳酸的产量，从而得出三种氘标记葡萄糖探针在肿瘤细胞中的消耗速度以及代谢物的生成速度等动态代谢特征．结果显示在细胞实验中三种氘标记葡萄糖均能够显示出肿瘤细胞的代谢特征，且葡萄糖的消耗速度并无显著性差异，氘标记水和氘标记乳酸的产量也符合理论估算．因此本文认为低成本的氘标记葡萄糖探针[2,3,4,6,6’-²H₅]-葡萄糖具备更好的临床转化价值．

关键词: 磁共振氘成像, 氘标记葡萄糖, 脑胶质瘤细胞, 核磁共振氘谱

Abstract:

Deuterium (²H) magnetic resonance imaging is an emerging molecular metabolic imaging method that can visualize the metabolic pathway in vivo, and therefore has great potential in clinical applications such as cancer detection. In this work, we aimed to compare the metabolic characteristics of three different deuterium-labeled glucose, namely [6,6’-²H₂]-glucose, [2,3,4,6,6’-²H₅]-glucose, and [1,2,3,4,5,6,6’-²H₇]-glucose through glioma cell experiment. The rat glioma C6 cells were incubated with three deuterium-labeled glucose, and cell media samples were collected at different time points, and underwent magnetic resonance deuterium spectroscopy to obtain the glucose consumption and the production of downstream metabolites, such as water and lactate, at each time point. The results showed that all three kinds of deuterium-labeled glucose probes were able to demonstrate the characteristic of tumor metabolism, and there was no significant difference in the consumption rate of the three kinds of deuterium-labeled glucose probes, and the production of the deuterium-labeled water and deuterium-labeled lactate were consistent with the theoretical estimation. Therefore, this paper concludes that the cost-effective deuterium-labeled glucose probe [2,3,4,6,6’-²H₅]-glucose has great clinical translational values.

Key words: deuterium magnetic resonance imaging, deuterated glucose, glioma cells, ²H NMR

中图分类号:

R445.2

方羿, 万倩, 袁家文, 林绍强, 李烨, 刘新, 郑海荣, 邹超. 三种氘标记的葡萄糖在大鼠脑胶质瘤细胞中的代谢特征对比[J]. 波谱学杂志, 2023, 40(3): 239-245.

FANG Yi, WAN Qian, YUAN Jiawen, LIN Shaoqiang, LI Ye, LIU Xin, ZHENG Hairong, ZOU Chao. Comparison Study of the Metabolic Characteristics of Three Kinds of Deuterium-labeled Glucose in Rat Glioma Cells[J]. Chinese Journal of Magnetic Resonance, 2023, 40(3): 239-245.

图/表 3

参考文献 27

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三种氘标记的葡萄糖在大鼠脑胶质瘤细胞中的代谢特征对比

Comparison Study of the Metabolic Characteristics of Three Kinds of Deuterium-labeled Glucose in Rat Glioma Cells

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