NMR 分析胶质瘤细胞系CHG5 和U87 的代谢轮廓

波谱学杂志

NMR 分析胶质瘤细胞系CHG5 和U87 的代谢轮廓

邵巍1，顾金苹1，黄彩华2，黄子成1，季天海1*，林东海1*

1. 厦门大学附属成功医院，厦门大学化学生物学系生物核磁共振实验室，福建厦门 361005；
2. 福建医科大学运动和康复研究所，福建福州 351009

收稿日期:2013-09-01 修回日期:2013-10-13 出版日期:2014-03-05 发布日期:2014-03-05
作者简介:邵巍（1983-），女，博士，助理研究员，主要从事基于核磁共振的肿瘤细胞代谢组学研究．电话：0592-6335744，E-mail: shw6610@ gmail.com. *通讯联系人：季天海，电话：0592-6335844，E-mail: kysea-ji@sina.com；林东海，电话：0592-2186078，E-mail: dhlin@xmu.edu.cn.
基金资助:
厦门市科技计划社会发展资助项目(3502Z20124019, 3502Z20104015)，福建省自然科学基金青年创新资助项目(2012D057)．

Metabolic Profiles of CHG5 and U87 Glioma Cell Lines Derived by 1H NMR

SHAO Wei1，GU Jin-ping1，HUANG Cai-hua2，HUANG Zi-cheng1，JI Tian-hai1*，LIN Dong-hai1*

1. Chenggong Hospital and College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
2. Research institute of Exercise and Rehabilitation, Fujian Medical University, Fuzhou 351009, China

Received:2013-09-01 Revised:2013-10-13 Online:2014-03-05 Published:2014-03-05
About author:*Corresponding author：JI Tian-hai, Tel: 0592-6335844, E-mail: kysea-ji@sina.com; LIN Dong-hai, Tel: 0592-2186078,E-mail: dhlin@xmu.edu.cn.
Supported by:
厦门市科技计划社会发展资助项目(3502Z20124019, 3502Z20104015)，福建省自然科学基金青年创新资助项目(2012D057)．

摘要/Abstract

摘要：

胶质瘤是中枢神经系统最常见的恶性肿瘤，其发病原因和恶性进展机制均不明确，给胶质瘤的临床诊疗带来很大困难．胶质瘤恶性进展伴随着癌细胞代谢改变，但可以反映胶质瘤恶性进展的代谢物信息和分子机制目前仍不清楚．该研究以两种不同恶性程度的胶质瘤细胞系CHG5 和U87 为研究对象，用基于NMR 的代谢组学方法分析这两种胶质瘤细胞系的代谢轮廓，寻找差异性代谢物．结果表明CHG5 和U87 的代谢轮廓存在明显差异．在高恶性程度的U87 细胞中，柠檬酸盐(citrate)等15 种代谢物有上升趋势；而乳酸盐(lactate)、牛磺酸(taurine)等6 种代谢物有下降趋势．以上结果表明这些差异性代谢物可能与胶质瘤细胞的恶性特性密切相关．这些从胶质瘤细胞系获取的代谢信息将成为临床标本代谢组学研究的重要前提和有益补充．

关键词: 核磁共振(NMR), 代谢轮廓, 差异性代谢物, 胶质瘤细胞, 恶性程度

Abstract:

Gliomas are the most common malignant tumors. The lack of detailed information on the progression of gliomas make their clinical diagnosis and treatment a challenging endeavor. In this study, the metabolic profiles of two glioma cell lines (i.e., CHG5 and U87) with different malignancy were analyzed by 1H NMR. The results showed that there were significant differences in the metabolic profiles of these two cell lines. High levels of 15 metabolites including citratate, and lower levels of 6 metabolites including lactate and taurine were found in the CHG5 cell line, compared to the U87 cell line. The
findings of this study suggested that metabolic profiles of glioma cell lines could be associated with their malignant features, thus may potentially be used as a measure in elucidating the molecular mechanisms underlying the progression of gliomas and in the development of non-invasive markers for monitoring of gliomas.

Key words: 1H NMR, metabolic profile, metabolite, glioma cell lines, malignant features

中图分类号:

O482.53

邵巍1，顾金苹1，黄彩华2，黄子成1，季天海1*，林东海1*. NMR 分析胶质瘤细胞系CHG5 和U87 的代谢轮廓[J]. 波谱学杂志.

SHAO Wei1，GU Jin-ping1，HUANG Cai-hua2，HUANG Zi-cheng1，JI Tian-hai1*，LIN Dong-hai1*.

Metabolic Profiles of CHG5 and U87 Glioma Cell Lines Derived by 1H NMR

[J]. Chinese Journal of Magnetic Resonance.

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