超极化129Xe磁共振分子影像学

波谱学杂志

超极化¹²⁹Xe磁共振分子影像学

张继^1,2，罗晴¹，郭茜旎¹，陈世桢¹，周欣^1*

1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心(中国科学院武汉物理与数学研究所)，湖北武汉 430071;
2. 中国科学院大学，北京 100049

收稿日期:2012-09-07 修回日期:2012-11-20 出版日期:2013-06-05 发布日期:2013-06-05
作者简介:张继（1988-），男，湖北人，硕士研究生，主要研究方向为超灵敏磁共振分子探针与分子影像学. E-mail: zhangji198805@gmail.com. *通讯联系人：周欣，电话：02787198802,E-mail: xinzhou@wipm.ac.cn.
基金资助:
国家自然科学基金资助项目(81227902, 11004228)；国家科学技术部创新方法资助项目（2010IM- 030600）；中国科学院知识创新工程重要方向资助项目（KJCX2-EW-N06）；中国科学院“百人计划”资助项目([2010]88).

Magnetic Resonance Molecular Imaging with Hyperpolarized ¹²⁹Xe

ZHANG Ji^1,2, LUO Qing¹, GUO Qian-ni¹, CHEN Shi-zhen¹, ZHOU Xin^1*

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-09-07 Revised:2012-11-20 Online:2013-06-05 Published:2013-06-05
About author:*Corresponding author: Zhou Xin,Tel:027-87198802,E-mail: xinzhou@wipm.ac.cn.
Supported by:
国家自然科学基金资助项目(81227902, 11004228)；国家科学技术部创新方法资助项目（2010IM- 030600）；中国科学院知识创新工程重要方向资助项目（KJCX2-EW-N06）；中国科学院“百人计划”资助项目([2010]88).

摘要/Abstract

摘要：

磁共振分子影像学发展的主要瓶颈之一在于灵敏度的限制，基于激光光泵和自旋交换技术能获得增强4~5个量级的超极化¹²⁹Xe磁共振信号，因此超极化¹²⁹Xe磁共振分子影像学相对于传统MRI在灵敏度上表现出巨大的优势. 围绕提高灵敏度这一核心MRI问题及其在科学研究中的应用，该文介绍了目前基于超极化¹²⁹Xe的生物分子探针的基本结构和原理，阐述了与之相关的分子影像学方法和技术，同时评述了当前的最新研究进展和发展方向.

关键词: 磁共振成像(MRI), 分子影像学, 灵敏度, 超极化¹²⁹Xe, 分子探针

Abstract:

The applications of magnetic resonance molecular imaging are often hindered by low sensitivity. Magnetic resonance signal of hyperpolarized ¹²⁹Xe can be enhanced 4~5 orders of magnitude by spin-exchange optical pumping. Ultrasensitive ¹²⁹Xe magnetic resonance molecular imaging shows a huge advantage in sensitivity comparing with the conventional MRI. In this paper, the improvement of the sensitivity of MRI and its applications to scientific research are introduced; the basic structures and principle of Xe-based biosensors are interpreted; the updated methodology and technology of molecular imaging are reviewed, and the advance of magnetic resonance molecular imaging with hyperpolarized ¹²⁹Xe is commented.

Key words: MRI, molecular imaging, hyperpolarized ¹²⁹Xe, sensitivity, molecular sensors

中图分类号:

O482. 53

张继1,2，罗晴1，郭茜旎1，陈世桢1，周欣1*. 超极化¹²⁹Xe磁共振分子影像学[J]. 波谱学杂志.

ZHANG Ji1,2, LUO Qing1, GUO Qian-ni1, CHEN Shi-zhen1, ZHOU Xin1*. Magnetic Resonance Molecular Imaging with Hyperpolarized ¹²⁹Xe[J]. Chinese Journal of Magnetic Resonance.

参考文献

[1] Wang Qian-feng(王前锋), Li Jian-qi（李建奇）, Wu Dong-mei（吴东梅）, et al. High-resolution diffusion-weighted imaging on small animals on a clinical 3 T MRI scanner（小动物高分辨扩散加权成像在临床MRI上的实现）[J]. Chinese J Magn Reson(波谱学杂志), 2012, 29(3): 372-378.

[2] Zhang Xiaodong(张晓东). The preliminary fMRI investigation of 10 Hz modulation laser acupuncture induced cerebral cortical activation in human(以功能性核磁共振造影初探10 Hz调制激光针灸刺激所引发人类大脑皮质的活化现象)[J]. Chinese J Magn Reson(波谱学杂志), 2010, 27(3): 369-378. 

[3] Weisslede R, Mahmood U. Molecular imaging[J]. Radiology, 2001, 219(2): 316-333．

[4] Zhou X. Hyperpolarized Noble Gases as Contrast Agents: In vivo NMR Imaging: Methods and Protocols[M]. USA: Humana Press, 2011.

[5] Zeng X, Wu Z, Call T, et al. Experimental determination of the rate constants for spin exchange between optically pumped K, Rb, and Cs atoms and ¹²⁹Xe nuclei in alkali metalnoblegas van der Waals molecules\[J\]. Phys Rev A, 1985, 31(1): 260-278.

[6] Bowers C R, Weitekamp D P. Transformation of symmertrization order to nuclear-spin magnetization by chemical-reaction and nuclear-magnetic-resonance[J]. Phys Rev Lett, 1986, 57(21): 2 645-2 648.

[7] Golman K, Axelsson O, Jóhannesson H, et al. Parahydrogen-induced polarization in imaging: subsecond C-13 angiography[J]. Magn Reson Med, 2001, 46(1): 1-5.

[8] Song C, Hu K N, Joo C G, et al. TOTAPOL: A biradical polarizing agent for dynamic nuclear polarization experiment in aqueous media[J]. J Am Chem Soc, 2006, 128(35): 11 385-11 390.

[9] Jan H, Larsen A, Fridlund B, et al. Increase in signal-to-noise ratio of >10,000 times in liquid-state NMR[J]. Proc Natl Acad Sci USA, 2003, 100(18): 10 158-10 163.

[10] Jameson C J. Multinuclear NMR[M]. New York: Plenum Press, 1987.

[11] Dybowski C, Bansal N, Duncan T M. NMR spectroscopy of xenon in confined spaces: clathrates, intercalates, and zeolites[J]. Annu Rev Phys Chem, 1991, 42(1): 33-464.

[12 Raftery D, Chmelka B F. Xenon NMR Spectroscopy, in NMR Basic Principles and Progress[M]. Berlin: Springer-Verlag, 1994.

[13] Schoenborn B P, Watson H C, Kendrew J C. Binding of xenon to sperm whale myoglobin[J]. Nature, 1965, 207(4992): 28-30. 

[14] Schoenborn B P, Nobbs C L. The binding of xenon to sperm whale deoxymyoglobin[J]. Mol Pharmacol, 1966, 2(5): 495-498.

[15] Schoenborn B P. Structure of alkaline metmyoglobin-xenon complex[J]. J Mol Biol, 1969, 45(2): 297-303. 

[16] Schoenborn B P. Binding of xenon to horse hemoglobin[J]. Nature, 1965, 208(5012): 760-762. 

[17] Tilton R F, Singh U C Jr., Weiner S J, et al. Computational studies of the interaction of myoglobin and xenon[J]. J Mol Biol, 1986, 192(2): 443-456. 

[18] Tilton R T Jr., Kuntz I D Jr.. Nuclear magnetic resonance studies of xenon-129 with myoglobin and hemoglobin[J]. Biochemistry, 1982, 21(26): 6 850-6 857.

[19] Locci E, Dehouck Y, Casu M, et al. Probing proteins in solution by ¹²⁹Xe NMR spectroscopy[J]. J Magn Reson, 2001, 150(2): 167-174.

[20] Spence M M, Rubin S M, Dimitrov I E, et al. Functionalized xenon as a biosensor[J]. Proc Natl Acad Sci USA, 2001, 98(19): 10 654-10 657.

[21] Sun Xian-ping(孙献平), Han Ye-qing(韩叶清), Luo Qing(罗晴), et al. Hyperpolarized ¹²⁹Xe magnetic resonance imaging and its applications in biomedicine(超极化¹²⁹Xe磁共振波谱和成像及在生物医学中的应用)[J]. Physics(物理), 2011, 40(6): 381-390.

[22] Bartik K, Luhmer M, Dutasta J P, et al. Xe-129 and H-1 NMR study of the reversible trapping of xenon by cryptophane-A in organic solution[J]. J Am Chem Soc, 1998, 120(4): 784-791.

[23] Bifone A, Song Y Q, Seydoux R, et al. NMR of Laser-polarized Xenon in human blood[J]. Proc Natl Acad Sci USA, 1996, 93(23): 12 932-12 936.

[24] Hill P A, Qian W, Roderic G E, et al. Thermodynamics of xenon binding to cryptophane in water and human plasma[J]. J Am Chem Soc, 2007, 129(30): 9 262-9 263.

[25] Meldrum T, Schr-der L, David E, et al. Xenon-based molecular sensors in lipid suspensions[J]. J Magn Reson, 2010, 205(2): 242-246.

[26] Rubin S, Spence M M, Dimitrov I, et al. Detection of a conformational change in maltose binding protein by ¹²⁹Xe NMR spectroscopy[J]. J Am Chem Soc, 2001, 123(35): 8 616-8 617.

[27] Schrder L, Lowery T, Hilty C, et al. Molecular imaging using a targeted magnetic resonance hyperpolarized biosensor\[J\]. Science, 2006, 314(5798): 446-449.

[28] Darzac M, Brotin T, Bouchu D, et al. Cryptophanols, new versatile compounds for the synthesis of functionalized cryptophanes and polycryptophanes[J]. Chem Commun, 2002, 7(1): 48-49. 

[29] Huber G, Brotin T, Dubois L, et al. Water soluble cryptophanes showing unprecedented affinity for xenon: Candidates as NMR-based biosensors[J]. J Am Chem Soc, 2006, 128(18): 6 239-6 246.

[30] Fogarty H A, Berthault P, Brotin T, et al. A cryptophane core optimized for xenon encapsulation[J]. J Am Chem Soc, 2007, 129(34): 10 332-10 333.

[31] Fairchild R M, Joseph A I, Holman K T, et al. A water-soluble Xe@cryptophane-111 complex exhibits very high thermodynamic stability and a peculiar Xe-129 NMR chemical shift[J]. J Am Chem Soc, 2010, 132(44): 15 505-15 507.

[32] Lowery T J, Garcia S, Ruiz E J, et al. Optimization of xenon biosensors for detection of protein interactions[J]. Chem Bio Chem, 2006, 7(1): 65-73.

[33] Seward G K, Qian W, Dmochowski I J. Peptide-mediated cellular uptake of cryptophane[J]. Bioconjugate Chem, 2008, 19(11): 2 129-2 135.

[34] Ye Y P, Bloch S, Xu B G, et al. Design, synthesis, and evaluation of near infrared fluorescent multimeric RGD peptides for targeting tumors[J]. J Med Chem, 2006, 49(7): 2 268-2 275.

[35] Seward G K, Bai Y B, Najat S, et al. Cell-compatible, integrin-targeted cryptophane ¹²⁹Xe NMR biosensors[J]. Chem Sci, 2011, 2(6): 1 103-1 110.

[36] Boutin C, Stopin A, Lenda F, et al. Cell uptake of a biosensor detected by hyperpolarized ¹²⁹Xe NMR the transferrin case[J]. Bioorgan Med Chem, 2011, 19(13): 4 135-4 143.

[37] (a) Patyal B R, Gao J H, Williams R F, et al. Longitudinal relaxation and diffusion measurements using magnetic resonance signals from laser-hyperpolarized Xe-129 nuclei[J]. 1997, 126(1): 58-65; (b) Ruppert K, Brookeman J R, Hagspiel K D, et al. Probing lung physiology with xenon polarization transfer contrast (XTC)[J]. Magn Reson Med, 2000, 44(3): 349-357.

[38] Spence M M, Ruiz E J, Rubin S M, et al. Development of functionalized xenon biosensor[J]. J Am Chem Soc, 2004, 126(46): 15 287-15 294.

[39] Moulè A J, Spence M M, Han S I, et al. Amplification of xenon NMR and MRI by remote detection[J]. Proc Natl Acad Sci USA, 2003, 100(16): 9 122-9 127.

[40] Zhou X, Graziani D, Pines A. Hyperpolarized xenon NMR and MRI signal amplification by gas extraction[J]. Proc Natl Acad Sci USA, 2009, 106(40): 16 903-16 906.

[41] Mynar J L, Lowery T J, Wemmer D E, et al. Xenon biosensor amplification via dendrimer-cage supramolecular constructs[J]. J Am Chem Soc, 2006, 128(19): 6 334-6 335.

[42] Meldrum T, Seim K L, Bajaj V S, et al. A xenon-based molecular sensor assembled on an MS2 viral capsid scaffold[J]. J Am Chem Soc, 2010, 132(17): 5 936-5 937.

[43] Schrder L, Meldrum T, Smith M, et al. Temperature response of ¹²⁹Xe depolarization transfer and its application for ultrasensitive NMR detection[J]. Phys Rev Lett, 2008, 100(25): 257 603(1-4).

[44] Berthault P, Huber G, Desvaux H. Biosensing using laser-polarized xenon NMR/MRI[J]. Prog Nuc Magn Reson Spectr, 2009, 55(1): 35-60.

[45] Qian W, Seward G K, Hill P A, et al. Designing ¹²⁹Xe NMR biosensors for matrix metalloproteinase detection[J]. J Am Chem Soc, 2006, 128(40): 13 274-13 283.

[46] Chambers J M, Hill P A, Aaron J A, et al. Cryptophane Xenon-129 nuclear magnetic resonance biosensors targeting human carbonic anhydrase[J]. J Am Chem Soc, 2009, 131(2): 563-569.

[47] Aaron J A, Chambers J M, Jude K M, et al. Structure of ¹²⁹Xe-cryptophane biosensor complexed with human carbonic anhydrase II[J]. J Am Chem Soc, 2008, 130(22): 6 942-6 943.

[48] Roy V, Brotin T, Dutasta J P, et al. A cryptophane biosensor for detection of specific nucleotide targets through xenon-NMR[J]. Chem Phys Chem, 2007, 8(14): 2 082-2 085.

[49] Schrder L, Chavez L, Meldrum T, et al. Temperature-controlled molecular depolarization gates in nuclear magnetic resonance[J]. Angew Chem Int Ed, 2008, 47(23): 4 316-4 320.

[1]	魏国境, 易佩伟, 陶泉, 冯衍秋. CEST成像不同量化方式在急性帕金森氏病小鼠模型研究中的应用比较[J]. 波谱学杂志, 2019, 36(2): 195-207.
[2]	黄朝晖, 张志, 陈黎, 陈俊飞, 张震, 陈方, 刘朝阳. MRI梯度预加重模块的分时复用设计[J]. 波谱学杂志, 2018, 35(4): 465-474.
[3]	刘颖, 宋明辉, 王坤, 章浩伟. 基于全可编程SoC和LabVIEW的磁共振接收系统设计[J]. 波谱学杂志, 2018, 35(4): 475-485.
[4]	汪红志, 赵地, 杨丽琴, 夏天, 周皛月, 苗志英. 基于AI+MRI的影像诊断的样本增广与批量标注方法[J]. 波谱学杂志, 2018, 35(4): 447-456.
[5]	陈海燕, 赵世龙, 李晓南, 刘国强, 胡丽丽, 刘弢. 低场永磁体磁共振射频场映像[J]. 波谱学杂志, 2018, 35(4): 498-504.
[6]	陶泉, 易佩伟, 魏国境, 冯衍秋. 基于CEST机制的pH成像方法、原理和应用[J]. 波谱学杂志, 2018, 35(4): 505-519.
[7]	翟国强, 张苗, 薄斌仕, 王乙, 范明霞, 李建奇. 基于复值和模值的肝脏磁共振水脂分离组合算法[J]. 波谱学杂志, 2018, 35(4): 417-426.
[8]	张波, 谢海滨, 严序, 李文静, 杨光. 旋转不变的非局域均值算法在磁共振图像去噪中的应用[J]. 波谱学杂志, 2018, 35(2): 162-169.
[9]	崔妍, 肖亮. 磁共振成像谱仪B₀信号的高精度发生与测试[J]. 波谱学杂志, 2018, 35(2): 170-177.
[10]	孙炜航, 孙钰, 汪丰, 王超洪, 杨涛. 变延迟进动定制激发(DANTE)序列在工程中的优化设计[J]. 波谱学杂志, 2018, 35(2): 150-161.
[11]	马芸, 郭虹, 王秋实, 张伟国, 吴昊. 基于影像的形态学特征与胶质母细胞瘤特征分子表达的相关性研究[J]. 波谱学杂志, 2018, 35(1): 22-30.
[12]	黄丽洁, 宋阳, 赵献策, 谢海滨, 吴东梅, 杨光. 一种结合并行成像和压缩感知的快速磁共振成像新方法[J]. 波谱学杂志, 2018, 35(1): 31-39.
[13]	罗庆, 张成秀, 李文静, 郑慧, 谢海滨, 杨光. 一种新的磁共振图像处理流水线的设计与实现[J]. 波谱学杂志, 2018, 35(1): 40-51.
[14]	宋瑞, 何砚发, 张波. 一种永磁磁共振中磁体涡流场的测量方法[J]. 波谱学杂志, 2018, 35(1): 52-59.
[15]	李宇宙, 张喆, 陈泉荣, 郭华. 一种以超分辨率理论为基础的磁共振眼球成像方法[J]. 波谱学杂志, 2017, 34(4): 439-452.

超极化¹²⁹Xe磁共振分子影像学

Magnetic Resonance Molecular Imaging with Hyperpolarized ¹²⁹Xe

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价