大鼠粪样中几种糖类物质的结构确定

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (3): 361-371.

大鼠粪样中几种糖类物质的结构确定

田园^1,2，唐惠儒^1*

1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心（中国科学院武汉物理与数学研究所），武汉 430071； 
2. 中国科学院研究生院，北京 100049

收稿日期:2011-11-22 修回日期:2011-12-02 出版日期:2012-09-05 发布日期:2012-09-05
通讯作者: 唐惠儒,huiru.tang@wipm.ac.cn E-mail:huiru.tang@wipm.ac.cn
基金资助:
国家自然科学基金资助项目（20825520，21175149）.

Identification and Structural Determination of Saccharides in Rat Feces

TIAN Yuan^1,2, TANG Hui-Ru^1*

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

Received:2011-11-22 Revised:2011-12-02 Online:2012-09-05 Published:2012-09-05
Contact: TANG Hui-Ru,huiru.tang@wipm.ac.cn E-mail:huiru.tang@wipm.ac.cn
Supported by:
国家自然科学基金资助项目（20825520，21175149）.

摘要/Abstract

摘要：

粪样代谢组能够反映宿主和肠道菌群相互作用的共代谢信息，然而粪样中糖类物质的归属信息迄今仍不完善. 该研究使用1D NMR技术检测到大鼠粪样中木糖、阿拉伯糖、半乳糖等单糖以及由阿拉伯糖和木糖组成的寡聚糖. 在使用固相萃取（SPE）方法进行分离富集的基础上，结合使用2D NMR实验技术（¹H-¹H COSY，¹H-¹H TOCSY，¹H-¹³C HSQC，¹H-¹³C HMBC以及DOSY）确定了这些糖类物质的结构，并对其1H NMR和¹³C NMR谱峰进行了归属.

关键词: 核磁共振（NMR）, 固相萃取（SPE）, 2D NMR技术, 粪样, 低聚糖

Abstract:

Fecal metabonomic NMR analysis has been found to offer important information for the complex interactions between mammalian metabolisms and their gut microbiome. In this study, we set to identify and assign saccharides signal from the NMR spectra of rat fecal samples. C18 solid-phase extraction was used to extract the saccharides from the fecal samples. The identities of the saccharides was elucidated by atomic connectivity and diffusion coefficient data obtained from 2D NMR data including ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹³C HSQC, ¹H-¹³C HMBC and ¹H DOSY. The saccharides identified included xylose, arabinose, galactose and arabinoxylan.

Key words: NMR, solid-phase extraction (SPE), 2D NMR, fece, oligosaccharide

中图分类号:

O482.53

田园, 唐惠儒. 大鼠粪样中几种糖类物质的结构确定[J]. 波谱学杂志, 2012, 29(3): 361-371.

TIAN Yuan, TANG Hui-Ru. Identification and Structural Determination of Saccharides in Rat Feces[J]. Chinese Journal of Magnetic Resonance, 2012, 29(3): 361-371.

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