Nanodisc体系在膜蛋白结构与功能研究中的应用

摘要/Abstract

摘要：

膜蛋白是生物膜功能的主要执行者，在生物体内参与许多重要的生理过程. 但是，由于很难获得稳定均匀并且维持膜蛋白正确构象的膜模拟环境，膜蛋白研究远远滞后于水溶型蛋白. 磷脂纳米盘（Nanodisc）是由高密度脂蛋白发展而来的用于膜蛋白研究的新型类膜结构. VDAC-1, GPCRs和细胞色素P450s等膜蛋白已成功的与Nanodisc组装起来，并且被人们利用液体核磁共振(NMR)和表面等离子体共振(SPR)等方法对其进行了相关的结构和生化研究. 随着高密度脂蛋白自身的设计修饰和Nanodisc相关技术的发展，Nanodisc将为膜蛋白的研究提供更多重要的信息.

关键词: 液体核磁共振（NMR）, 膜蛋白, Nanodisc, 高密度脂蛋白

Abstract:

Membrane proteins are responsible for a wide range of essential physiological processes, and important drug targets for treating diseases. However, structural study on membrane proteins lags far behind that on soluble proteins, and this, in large part, is due to technical difficulties in preparing homogeneous, stable and structurally relevant samples in a membrane-like environment. Phospholipid bilayer Nanodisc is novel model membrane derived from highdensity lipoprotein particles, and has proven to be useful in structural studies on membrane proteins. It has been shown that Nanodisc can provide a “native-like” lipid bilayer environment for many membrane proteins, such as VDAC-1 (voltage-dependent anion channel), GPCRs (G-protein-coupled receptors) and cytochrome P450s, thus enabling structural studies on these membrane proteins with NMR/surface plasmon resonance (SPR) techniques. Furthermore, with more sophisticated techniques such as modification of membrane scaffold proteins (MSP), the use of Nanodisc may yield structural and functional insights into a wide variety of membrane proteins in a biologically relevant membrane environment.

Key words: NMR, membrane protein, Nanodisc, high-density lipoprotein

中图分类号:

O482.53
Q71

毕允晨, 王玉娟, 王俊峰. Nanodisc体系在膜蛋白结构与功能研究中的应用[J]. 波谱学杂志, 2011, 28(2): 177-189.

BI Yuan-Chen, WANG Yu-Juan, WANG Jun-Feng. The Nanodics: A Novel Tool to Study Membrane Protein Structure and Function[J]. Chinese Journal of Magnetic Resonance, 2011, 28(2): 177-189.

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