大鼠眼球的高场磁共振成像研究

波谱学杂志 ›› 2010, Vol. 27 ›› Issue (4): 630-634.

大鼠眼球的高场磁共振成像研究

安艳丽¹，张洪英^1，2*

1. 东南大学附属中大医院放射科，江苏省分子影像与功能影像重点实验室，江苏南京 210009； 
2. 江苏省苏北人民医院放射科，扬州大学临床医学院，江苏扬州 225001

收稿日期:2010-07-05 修回日期:2010-09-06 出版日期:2010-12-05 发布日期:2010-12-05
基金资助:
国家自然科学基金资助项目（30870704）.

MRI of Rat Eyeballs

AN Yan-Li¹, ZHANG Hong-Ying^1，2*

1. Jiang Su Key Laboratory of Molecular Imaging and Functional Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China;
2. Department of Radiology, Subei Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, China

Received:2010-07-05 Revised:2010-09-06 Online:2010-12-05 Published:2010-12-05
Supported by:
国家自然科学基金资助项目（30870704）.

摘要/Abstract

摘要：

该文旨在研究微型磁共振（MRI）对大鼠眼球的成像效果和应用. 通过对10只SD大鼠的20只眼球进行7.0 T MRI检查，应用常规T1WI和T2WI序列高分辨率扫描；观察MRI图像上大鼠眼球的结构，并比较MRI测量与组织学显微镜下测量视网膜厚度结果. 磁共振扫描清楚地显示了所有受试大鼠眼球的主要结构，包括角膜、晶状体、玻璃体、视网膜、巩膜、虹膜、睫状体、视神经. 球壁结构磁共振图像层次与组织学结构层次有良好对应性；磁共振视网膜厚度测量值与显微镜下视网膜厚度测量数据进行配对 t 检验，P＞0.05，二者无显著差异. 由此得出的结论是小动物MRI可以对大鼠眼球细微解剖结构进行无创性的成像，为我们提供了一个研究大鼠眼科疾病模型的形态学及功能变化的手段. 

关键词: 磁共振成像（MRI）, 大鼠, 眼球, 视网膜

Abstract:

Twenty eyeballs from 10 rats were scanned at 7 T with T₁-eighted imaging and T₂-weighted imaging sequences. The structures of eyeballs on the images were evaluated, and the retinal thickness was measured on T₂-weighted images. Most of the anatomical structures within the eyeballs could be distinguished on the images, including: cornea, lens crystalline, vitreous body, retina, sclera, iris and ciliary body, as well as the optic nerves. The thickness of retina determined with MRI was 209±20 μm, which was consistent with previous reports. There was no significant difference for the retinal thickness between MRI and the microscopic measurements. The findings from this -study- suggest that MRI can provide detailed anatomical imaging of rat eyeballs and optic nerve, and can be employed as a useful tool for investigating models of ophthalmological diseases.

Key words: MRI, rat, eyeball, retina

中图分类号:

R445.2

安艳丽, 张洪英. 大鼠眼球的高场磁共振成像研究[J]. 波谱学杂志, 2010, 27(4): 630-634.

AN Yan-Li, ZHANG Hong-Ying. MRI of Rat Eyeballs[J]. Chinese Journal of Magnetic Resonance, 2010, 27(4): 630-634.

参考文献

[1] Berkowitz B A, Roberts R, Penn J S, et al. High-resolution manganese-enhanced MRI of experimental retinopathy of prematurity[J]. Invest Ophthalmol Vis Sci, 2007, 48: 4 733-4 740.

[2] Berkowitz B A, Roberts R, Goebel D J, et al. Noninvasive and simultaneous imaging of layer-specific retinal functional adaptation by manganese-enhanced MRI[J]. Invest Ophthalmol Vis Sci, 2006, 47: 2 668-2 674.

[3] Luan H, Roberts R, Sniegowski M, et al. Retinal thickness and subnormal retinal oxygenation response in experimental diabetic retinopathy[J]. Invest Ophthalmol Vis Sci, 2006, 47: 320-328.

[4] Ito Y, Berkowitz B A. MR studies of retinal oxygenation[J]. Vision Res, 2001, 41: 1 307-1 311.

[5] Cheng H, Nair G, Walker T A, et al. Structural and functional MRI reveals multiple retinal layers[J]. Proc Natl Acad Sci USA, 2006, 103: 17 525-17 530.

[6] Berkowitz B A, Roberts R, Luan H, et al. Dynamic contrast-enhanced MRI measurements of passive permeability through blood retinal barrier in diabetic rats[J]. Invest Ophthalmol Vis Sci, 2004, 45: 2 391-2 398.

[7] Shen Q, Cheng H Y, Pardue M T, et al. Magnetic resonance imaging of anatomical and vascular layers of the cat retina[J]. J Magn Reson Imaging, 2006, 23: 465-472.

[8] Pop-Fanea L, Vallespin S N, Hutchison J M, et al. Evaluation of MRI for in vivo monitoring of retinal damage and detachment in experimental ocular inflammation[J]. Magn Reson Med, 2005, 53: 61-68.

[9] Thuen M, Singstad T E, Pedersen T B, et al. Manganese-enhanced MRI of the optic visual pathway and optic nerve injury in adult rats[J]. J Magn Reson Imaging, 2005, 22: 492-500.

[10] Watanabe T, Michaelis T, Frahm J. Mapping of retinal projections in the living rat using high-resolution 3D gradient-echo MRI with Mn²⁺induced contrast[J]. Magn Reson Med, 2001, 46: 424-429.

[1]	魏国境, 易佩伟, 陶泉, 冯衍秋. CEST成像不同量化方式在急性帕金森氏病小鼠模型研究中的应用比较[J]. 波谱学杂志, 2019, 36(2): 195-207.
[2]	黄朝晖, 张志, 陈黎, 陈俊飞, 张震, 陈方, 刘朝阳. MRI梯度预加重模块的分时复用设计[J]. 波谱学杂志, 2018, 35(4): 465-474.
[3]	刘颖, 宋明辉, 王坤, 章浩伟. 基于全可编程SoC和LabVIEW的磁共振接收系统设计[J]. 波谱学杂志, 2018, 35(4): 475-485.
[4]	汪红志, 赵地, 杨丽琴, 夏天, 周皛月, 苗志英. 基于AI+MRI的影像诊断的样本增广与批量标注方法[J]. 波谱学杂志, 2018, 35(4): 447-456.
[5]	陈海燕, 赵世龙, 李晓南, 刘国强, 胡丽丽, 刘弢. 低场永磁体磁共振射频场映像[J]. 波谱学杂志, 2018, 35(4): 498-504.
[6]	陶泉, 易佩伟, 魏国境, 冯衍秋. 基于CEST机制的pH成像方法、原理和应用[J]. 波谱学杂志, 2018, 35(4): 505-519.
[7]	翟国强, 张苗, 薄斌仕, 王乙, 范明霞, 李建奇. 基于复值和模值的肝脏磁共振水脂分离组合算法[J]. 波谱学杂志, 2018, 35(4): 417-426.
[8]	张波, 谢海滨, 严序, 李文静, 杨光. 旋转不变的非局域均值算法在磁共振图像去噪中的应用[J]. 波谱学杂志, 2018, 35(2): 162-169.
[9]	崔妍, 肖亮. 磁共振成像谱仪B₀信号的高精度发生与测试[J]. 波谱学杂志, 2018, 35(2): 170-177.
[10]	孙炜航, 孙钰, 汪丰, 王超洪, 杨涛. 变延迟进动定制激发(DANTE)序列在工程中的优化设计[J]. 波谱学杂志, 2018, 35(2): 150-161.
[11]	马芸, 郭虹, 王秋实, 张伟国, 吴昊. 基于影像的形态学特征与胶质母细胞瘤特征分子表达的相关性研究[J]. 波谱学杂志, 2018, 35(1): 22-30.
[12]	黄丽洁, 宋阳, 赵献策, 谢海滨, 吴东梅, 杨光. 一种结合并行成像和压缩感知的快速磁共振成像新方法[J]. 波谱学杂志, 2018, 35(1): 31-39.
[13]	罗庆, 张成秀, 李文静, 郑慧, 谢海滨, 杨光. 一种新的磁共振图像处理流水线的设计与实现[J]. 波谱学杂志, 2018, 35(1): 40-51.
[14]	宋瑞, 何砚发, 张波. 一种永磁磁共振中磁体涡流场的测量方法[J]. 波谱学杂志, 2018, 35(1): 52-59.
[15]	李宇宙, 张喆, 陈泉荣, 郭华. 一种以超分辨率理论为基础的磁共振眼球成像方法[J]. 波谱学杂志, 2017, 34(4): 439-452.