葛根素-7-磷酸二乙酯的NMR研究

波谱学杂志 ›› 2005, Vol. 22 ›› Issue (4): 409-414.

葛根素-7-磷酸二乙酯的NMR研究

河南省化学生物与有机化学重点实验室，郑州大学化学系，河南郑州 450052

收稿日期:2005-05-11 修回日期:2005-07-06 出版日期:2005-12-05 发布日期:2005-12-05
基金资助:
国家自然科学基金（20472076）和河南省杰出青年基金资助项目（0512001400）.

Synthesis and NMR Characterization of Diethyl Puerarin-7-yl Phosphate

Henan Provincial Key Laboratory of Bio- Chemistry and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China

Received:2005-05-11 Revised:2005-07-06 Online:2005-12-05 Published:2005-12-05
Supported by:
国家自然科学基金（20472076）和河南省杰出青年基金资助项目（0512001400）.

摘要/Abstract

摘要：

葛根素是从常用中药野葛根中分离提取出来的一种具有很强生物活性的异黄酮碳苷类化合物，设计并合成了一种新的化合物葛根素-7-磷酸二乙酯. 在常温下，这种新的化合物在NMR谱上有两组相关的信号峰，即在溶液中以两种不同的构象存在. 本文利用HSQC和HMBC等多种二维核磁共振技术对这种化合物的NMR谱进行了全归属.

关键词: NMR, 全归属, 2D NMR, 葛根素-7-磷酸二乙基酯

Abstract:

Puerarin, an isoflavone compound, is the bioactive component of traditional Chinese medicine puerarin lobate (wild). A novel diethyl puerarin-7-yl phosphate was synthesized by the Atherton-Todd reaction with high productive yield. It was found that the compound synthesized has two sets of NMR signals at room temperature, suggesting the existence of two conformational isomers in solution. The ¹H chemical shifts of the compound were assigned using two-dimensional NMR techniques, including ¹H-detected heteronuclear multiple-quantum coherence and ¹H-detected multiple-bond heteronuclear multiple-quantum coherence.

Key words: NMR, chemical shift assignment, diethyl puerarin-7-yl phosphate, isoflavone

中图分类号:

O641

袁金伟, 陈晓岚, 汪茂田, 屈凌波, 赵玉芬. 葛根素-7-磷酸二乙酯的NMR研究[J]. 波谱学杂志, 2005, 22(4): 409-414.

YUAN Jin-Wei, CHEN Xiao-Lan, WANG Mao-Tian, QU Ling-Bo, ZHAO Yu-Fen. Synthesis and NMR Characterization of Diethyl Puerarin-7-yl Phosphate[J]. Chinese Journal of Magnetic Resonance, 2005, 22(4): 409-414.

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