电刺激诱发大鼠慢性颞叶癫痫模型的磁共振成像和波谱特征的研究

波谱学杂志 ›› 2004, Vol. 21 ›› Issue (3): 281-288.

电刺激诱发大鼠慢性颞叶癫痫模型的磁共振成像和波谱特征的研究

杨运煌^1*，臧颖²，刁芳明²，韩丹²，李丽云¹，叶朝辉¹，刘买利¹

1.波谱与原子分子物理国家重点实验室(中国科学院武汉物理与数学研究所)，湖北武汉 430071; 
2.武汉大学医学院，生理学教研室，湖北武汉 430071

收稿日期:2004-02-11 修回日期:2004-04-16 出版日期:2004-09-05 发布日期:2004-09-05
基金资助:
国家自然科学基金重点项目（10234070）、面上项目（20175035）资助.

CEREBRAL CHANGES DURING THE EARLY STAGE OF A RAT MODEL OF CHRONIC TEMPORAL EPILEPSY OBSERVED BY MAGNETIC RESONANCE IMAGING [STHZ]AND LOCALIZED IN VIVO ¹H MRS

YANG Yun-Huang^1*, ZHANG Ying², DIAO Fang-Ming², HAN Dan², LI Li-Yun¹, YE Chao-Hui¹, LIU Mai-Li¹

1.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics（Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences）, Wuhan 430071,China；
2.Department of Physiology, Medical Academy, Wuhan University, Wuhan 430071, China

Received:2004-02-11 Revised:2004-04-16 Online:2004-09-05 Published:2004-09-05
Supported by:
国家自然科学基金重点项目（10234070）、面上项目（20175035）资助.

摘要/Abstract

摘要：

应用磁共振成像(MRI)和质子定域波谱(¹H-MRS)对强直流电刺激大鼠基树突区诱发慢性颞叶癫痫模型进行研究. MRI实验表明：随着强直流电刺激时间的延长，在T₂加权像(T₂-MRI)中，模型大鼠的海马区腹、中侧区单侧或双侧，呈现异常高信号，扩散加权像(DWI)信号呈低信号，质子密度像无明显改变, 表明T₂值和表观扩散系数(ADC) 值较大的自由水比例增大. 磁共振波谱实验发现：模型大鼠T₂-MRI中信号异常区与其对侧区的¹H-MRS相比，NAA，PCr（包括Cr）和Cho的峰面积均无显著改变，表明在慢性颞叶癫痫模型早期¹H-MRS不能检测到神经元损伤或死亡.

关键词: 磁共振成像, 颞叶癫痫, 定域活体磁共振氢谱, 大鼠

Abstract:

In this study, MRI and localized in vivo ¹H-MRS were applied to study cerebral changes during the early stage of a rat model of chronic temporal epilepsy. Epilepsy was induced by repetitive tetanus to the right basal dendrite region. Six days after the initial tetanus, T₂-weighted MRI (T-₂W-MRI) revealed that there was a significant increase in signal intensity of the right ventral part of hippocampus, the signal intensity of the same region decreased significantly in diffusion-weighted MRI (DWI). Such changes may be caused by the increase of the ratio of free water to bounded water in the affected region. Decrease of NAA was not found in the foci of epilepsy with localized in vivo ¹H-MRS, suggesting that there existed little or no loss of neurons during the early stage of this model of temporal epilepsy.

Key words: MRI, ¹H-MRS, temporal epilepsy, brain, rat

中图分类号:

O482.53

杨运煌, 臧颖, 刁芳明, 韩丹, 李丽云, 叶朝辉, 刘买利. 电刺激诱发大鼠慢性颞叶癫痫模型的磁共振成像和波谱特征的研究[J]. 波谱学杂志, 2004, 21(3): 281-288.

YANG Yun-Huang, ZHANG Ying, DIAO Fang-Ming, HAN Dan, LI Li-Yun, YE Chao-Hui, LIU Mai-Li. CEREBRAL CHANGES DURING THE EARLY STAGE OF A RAT MODEL OF CHRONIC TEMPORAL EPILEPSY OBSERVED BY MAGNETIC RESONANCE IMAGING [STHZ]AND LOCALIZED IN VIVO ¹H MRS[J]. Chinese Journal of Magnetic Resonance, 2004, 21(3): 281-288.

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