一种示踪干细胞的磁共振-荧光双模成像探针

doi:10.11938/cjmr20182659

波谱学杂志 ›› 2019, Vol. 36 ›› Issue (2): 127-137.doi: 10.11938/cjmr20182659

• 天眷波谱青年论坛特邀稿件 • 上一篇下一篇

一种示踪干细胞的磁共振-荧光双模成像探针

赵敏敏^1,2, 张艳辉², 张朋利², 谭波², 蒋海珍¹, 张海禄², 邓宗武²

1. 上海大学理学院, 上海 200444;
2. 中国科学院苏州纳米技术与纳米仿生研究所, 江苏苏州 215123

收稿日期:2018-06-06 发布日期:2018-07-31
通讯作者: 张海禄 Tel:+86-51-262872713, E-mail:hlzhang2008@sinano.ac.cn;邓宗武 Tel:+86-51-262872559, E-mail:zwdeng2007@sinano.ac.cn E-mail:hlzhang2008@sinano.ac.cn;zwdeng2007@sinano.ac.cn
作者简介:张海禄博士,中国科学院苏州纳米技术与纳米仿生研究所研究员,2008年博士毕业于中国科学院武汉物理与数学研究所.目前任江苏省核磁共振专业委员会副主任委员,中华放射学会磁共振物理与工程学组委员,中国晶体学会药物学专业委员会常务委员.他的研究领域主要为药物固态化学和纳米-生物界面物理.
基金资助:
the National Natural Science Foundation of China (31371010, 21673281); Funds from Youth Innovation Promotion Association of the Chinese Academy of Sciences (2012242); Key Research Project from the Ministry of Science and Technology of China (2017YFA0104300).

A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking

ZHAO Min-min^1,2, ZHANG Yan-hui², ZHANG Peng-li², TAN Bo², JIANG Hai-zhen¹, ZHANG Hai-lu², DENG Zong-wu²

1. College of Sciences, Shanghai University, Shanghai 200444, China;
2. Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

Received:2018-06-06 Published:2018-07-31
Supported by:
the National Natural Science Foundation of China (31371010, 21673281); Funds from Youth Innovation Promotion Association of the Chinese Academy of Sciences (2012242); Key Research Project from the Ministry of Science and Technology of China (2017YFA0104300).

摘要/Abstract

摘要： 本文设计并合成了Gd基磁共振-荧光双模成像探针——Gd-DOTA-PEG-GA，通过电穿孔的方式标记人源间充质干细胞（hMSCs）.电穿孔标记诱导细胞将探针组装成团簇状纳米粒子进入细胞质，显著延长其与细胞结合的时间，并呈现出明显的T₂信号减弱效应，且信号减弱效应可以持续7天以上.在水溶液中，该探针的发射带集中在498 nm，并且荧光强度在一周内无明显衰减.该探针标记的细胞在荧光倒置显微镜下呈现绿色荧光.这些结果表明该探针可以作为磁共振-荧光双模成像探针用于干细胞示踪.

关键词: 磁共振成像, 荧光成像, 双模探针, 人源间充质干细胞

Abstract: A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self-assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T₂-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo.

Key words: magnetic resonance imaging, fluorescent imaging, dual-mode probe, human mesenchymal stem cell

中图分类号:

O482.53

赵敏敏, 张艳辉, 张朋利, 谭波, 蒋海珍, 张海禄, 邓宗武. 一种示踪干细胞的磁共振-荧光双模成像探针[J]. 波谱学杂志, 2019, 36(2): 127-137.

ZHAO Min-min, ZHANG Yan-hui, ZHANG Peng-li, TAN Bo, JIANG Hai-zhen, ZHANG Hai-lu, DENG Zong-wu. A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 127-137.

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一种示踪干细胞的磁共振-荧光双模成像探针

A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking

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