Chinese Journal of Magnetic Resonance

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Metabolomic Analysis of Clostridium thermocellum

ZHU Xin-shu1,2,CUI Jia-tao1,2,FENG Yin-gang1,ZHANG Jing-tao1*,CUI Qiu1,3*


 
  

  1. 1. Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
  • Received:2013-05-30 Revised:2013-11-25 Online:2014-06-05 Published:2014-06-05
  • About author:*Corresponding author:ZHANG Jing-tao, Tel: 0532-80662705,E-mail:zhang_jt@qibebt.ac.cn;CUI Qiu, Tel: 0532-80662706,E-mail:cuiqiu@qibebt.ac.cn.
  • Supported by:

    国家重点基础研究发展计划资助项目(2011CB7074040),中国科学院科研装备研制项目(YZ201138),国家高技术研究发展计划资助项目(2011BAD22B02).

Abstract:

Clostridium thermocellum is a thermophilic anaerobic gram-positive bacteria that can efficiently utilize lignocelluloses. It is one of the most important candidate microorganisms for cellulosic ethanol production with consolidated bioprocessing. Metabolome reflects the interaction between cell and its environmental factors, and is an important component of system biology. In this work, 1H NMR and 2D NMR techniques were used to analysis the central metabolites of C. thermocellum. Some biologically significant metabolites were identified in the intracellular compartment of C. thermocellum, including such as cellodextrin, phosphoenolpyruvate and d-erythrose-4- phosphate. Preliminary multivariate statistical analysis of the wild type strain and ethanol tolerant strain showed various metabolic differences in several metabolic pathways including actively conversion of cellubiose to cellodextrin, enhancement of non-oxidative pentose phosphate pathway and suppression of Embden-Meyerhof-Parnas pathway.

Key words: liquid NMR, assignment, 2D NMR, Clostridium thermocellum, cellodextrin, metabolomics/metabonomics

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