MRI of Rat Eyeballs

Abstract

Abstract:

Twenty eyeballs from 10 rats were scanned at 7 T with T₁-eighted imaging and T₂-weighted imaging sequences. The structures of eyeballs on the images were evaluated, and the retinal thickness was measured on T₂-weighted images. Most of the anatomical structures within the eyeballs could be distinguished on the images, including: cornea, lens crystalline, vitreous body, retina, sclera, iris and ciliary body, as well as the optic nerves. The thickness of retina determined with MRI was 209±20 μm, which was consistent with previous reports. There was no significant difference for the retinal thickness between MRI and the microscopic measurements. The findings from this -study- suggest that MRI can provide detailed anatomical imaging of rat eyeballs and optic nerve, and can be employed as a useful tool for investigating models of ophthalmological diseases.

Key words: MRI, rat, eyeball, retina

CLC Number:

R445.2

AN Yan-Li, ZHANG Hong-Ying. MRI of Rat Eyeballs[J]. Chinese Journal of Magnetic Resonance, 2010, 27(4): 630-634.

References

[1] Berkowitz B A, Roberts R, Penn J S, et al. High-resolution manganese-enhanced MRI of experimental retinopathy of prematurity[J]. Invest Ophthalmol Vis Sci, 2007, 48: 4 733-4 740.

[2] Berkowitz B A, Roberts R, Goebel D J, et al. Noninvasive and simultaneous imaging of layer-specific retinal functional adaptation by manganese-enhanced MRI[J]. Invest Ophthalmol Vis Sci, 2006, 47: 2 668-2 674.

[3] Luan H, Roberts R, Sniegowski M, et al. Retinal thickness and subnormal retinal oxygenation response in experimental diabetic retinopathy[J]. Invest Ophthalmol Vis Sci, 2006, 47: 320-328.

[4] Ito Y, Berkowitz B A. MR studies of retinal oxygenation[J]. Vision Res, 2001, 41: 1 307-1 311.

[5] Cheng H, Nair G, Walker T A, et al. Structural and functional MRI reveals multiple retinal layers[J]. Proc Natl Acad Sci USA, 2006, 103: 17 525-17 530.

[6] Berkowitz B A, Roberts R, Luan H, et al. Dynamic contrast-enhanced MRI measurements of passive permeability through blood retinal barrier in diabetic rats[J]. Invest Ophthalmol Vis Sci, 2004, 45: 2 391-2 398.

[7] Shen Q, Cheng H Y, Pardue M T, et al. Magnetic resonance imaging of anatomical and vascular layers of the cat retina[J]. J Magn Reson Imaging, 2006, 23: 465-472.

[8] Pop-Fanea L, Vallespin S N, Hutchison J M, et al. Evaluation of MRI for in vivo monitoring of retinal damage and detachment in experimental ocular inflammation[J]. Magn Reson Med, 2005, 53: 61-68.

[9] Thuen M, Singstad T E, Pedersen T B, et al. Manganese-enhanced MRI of the optic visual pathway and optic nerve injury in adult rats[J]. J Magn Reson Imaging, 2005, 22: 492-500.

[10] Watanabe T, Michaelis T, Frahm J. Mapping of retinal projections in the living rat using high-resolution 3D gradient-echo MRI with Mn²⁺induced contrast[J]. Magn Reson Med, 2001, 46: 424-429.

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