Chinese Journal of Magnetic Resonance

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Metabolic Profiles of CHG5 and U87 Glioma Cell Lines Derived by 1H NMR

SHAO Wei1,GU Jin-ping1,HUANG Cai-hua2,HUANG Zi-cheng1,JI Tian-hai1*,LIN Dong-hai1*   

  1. 1. Chenggong Hospital and College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
    2. Research institute of Exercise and Rehabilitation, Fujian Medical University, Fuzhou 351009, China
  • Received:2013-09-01 Revised:2013-10-13 Online:2014-03-05 Published:2014-03-05
  • About author:*Corresponding author:JI Tian-hai, Tel: 0592-6335844, E-mail: kysea-ji@sina.com; LIN Dong-hai, Tel: 0592-2186078,E-mail: dhlin@xmu.edu.cn.
  • Supported by:

    厦门市科技计划社会发展资助项目(3502Z20124019, 3502Z20104015),福建省自然科学基金青年创新资助项目(2012D057).

Abstract:

Gliomas are the most common malignant tumors. The lack of detailed information on the progression of gliomas make their clinical diagnosis and treatment a challenging endeavor. In this study, the metabolic profiles of two glioma cell lines (i.e., CHG5 and U87) with different malignancy were analyzed by 1H NMR. The results showed that there were significant differences in the metabolic profiles of these two cell lines. High levels of 15 metabolites including citratate, and lower levels of 6 metabolites including lactate and taurine were found in the CHG5 cell line, compared to the U87 cell line. The
findings of this study suggested that metabolic profiles of glioma cell lines could be associated with their malignant features, thus may potentially be used as a measure in elucidating the molecular mechanisms underlying the progression of gliomas and in the development of non-invasive markers for monitoring of gliomas.

Key words: 1H NMR, metabolic profile, metabolite, glioma cell lines, malignant features

CLC Number: