Paramagnetic Relaxation Enhancement——A Tool for Visualizing Transient Protein Structures

Abstract

Abstract:

Paramagnetic relaxation enhancement (PRE) refers to acceleration of relaxation rates via dipole-dipole interactions between an unpaired electron and a nucleus. The theoretical framework of PRE was laid in the 1950s, yet its applications in studying protein structures only took place in the last decade. In this review, the theories underpinning PRE and the approaches to measure PRE rates are introduced. The commonly used paramagnetic probes for generating PRE in protein systems are reviewed. The principles of using PRE to characterize protein ground-state structure and transient excited-state structure are described. Further improvements of PRE techniques affording more detailed and comprehensive information regarding protein structure and dynamics are envisioned.

Key words: paramagnetic relaxation enhancement (PRE), protein dynamics, protein transient structure

CLC Number:

O482.53
Q71

LIU Zhu, TANG Chun. Paramagnetic Relaxation Enhancement——A Tool for Visualizing Transient Protein Structures[J]. Chinese Journal of Magnetic Resonance, 2011, 28(3): 301-316.

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