Chinese Journal of Magnetic Resonance ›› 2005, Vol. 22 ›› Issue (1): 113-121.

• Articles • Previous Articles    

Noninvasive Determination of Intracellular Free Mg2+ by 31P and 19F NMR: A Review

  

  1. Institute of Radiation Medicine,  Academy of Military Medical Sciences,  Beijing 100850,  China
  • Received:2004-06-24 Revised:2004-08-23 Online:2005-03-05 Published:2005-03-05
  • Supported by:

    国家自然科学基金资助项目(30271608); 北京市自然科学基金资助项目(7042048).

Abstract:

Magnesium ion (Mg2+) plays important roles in many biochemical reactions occurring in cells,  and determination of intracellular free Mg2+ concentration is essential for understanding the functioning of intact cells. Nuclear magnetic resonance (NMR),  a spectroscopic technique that measures magnetic nuclei and their interactions with the environment,  has gained considerable success and popularity in studying metal ions in vivo. 31P and 19F NMR can be used to measure intracellular concentration of free Mg2+,  and these techniques has the advantage of noninvasiveness so that free Mg2+ in the intracellular environment can be observed in essentially unperturbed living systems. In this review,  the theories and applications of 31P  and 19F NMR for determining intracellular concentration of free Mg2+ are discussed:.

Key words: 31P NMR,  19F NMR,  Mg2+,  noninvasive determination

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