延龄草中2 个甾体皂苷的2D NMR 研究

doi:10.11938/cjmr20150112

波谱学杂志 ›› 2015, Vol. 32 ›› Issue (1): 105-115.doi: 10.11938/cjmr20150112

延龄草中2 个甾体皂苷的2D NMR 研究

柴江1，王欣3，李慧敏4，张欣1，杨芳1，唐志书1，宋小妹1，裴月湖4，梅其炳2，岳正刚1,2?

1. 陕西中医学院，药学院，陕西省中药资源产业化协同创新中心，陕西省中药基础与新药研究重点实验室，陕西省风湿与肿瘤类中药制剂工程技术研究中心，陕西咸阳712046；
2. 第四军医大学，药学院，国家中药管理局中药胃肠药理国家重点研究室，秦巴山区中药发展协同创新中心，陕西西安 710032；
3. 西安市第一医院，陕西西安 710002；
4. 沈阳药科大学，中药学院，辽宁沈阳 110016

收稿日期:2014-05-26 修回日期:2015-01-12 出版日期:2015-03-05 发布日期:2015-03-05
作者简介:柴江(1989-)，男，陕西宝鸡人，硕士研究生，E-mail: chaijiang891989@126.com. *通讯联系人：岳正刚，电话：18092086211, E-mail: liuxingjian1981@163.com.
基金资助:
国家自然科学基金资助项目(81373978)；陕西省重点科技创新团队资助项目(2012KCT-20)；陕西省“13115”科技创新工程专项资助项目(2010ZDGC-22)；陕西省教育厅科技计划资助项目(09jk394)．

A Two-Dimensional NMR Study on Steroid Glycosides Extracted from Trillium tschonoskii

CHAI Jiang1，WANG Xin3，LI Hui-min4，ZHANG Xin1，YANG Fang1，TANG Zhi-shu1，SONG Xiao-mei1，PEI Yue-hu4，MEI Qi-bing2，YUE Zheng-gang1,2?

1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, Shannxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi Rheumatism and Tumor Center of TCM Engineering Technology Research, School of Pharmacy, Shannxi University of Traditional Chinese Medicine, Xianyang 712046, China;
2. Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Collaborative Innovation Center for Chinese Medicine in Qinba Mountains, School of Pharmacy, the Fourth Military Medical University, Xi′an 710032, China;
3. The First Hospital of Xi′an, Xi′an 710002, China;
4. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2014-05-26 Revised:2015-01-12 Online:2015-03-05 Published:2015-03-05
About author:*Corresponding author：YUE Zheng-gang, Tel: +86-18092086211, E-mail: liuxingjian1981@163.com.
Supported by:
国家自然科学基金资助项目(81373978)；陕西省重点科技创新团队资助项目(2012KCT-20)；陕西省“13115”科技创新工程专项资助项目(2010ZDGC-22)；陕西省教育厅科技计划资助项目(09jk394)．

摘要/Abstract

摘要：

采用柱色谱法从延龄草中分离得到两个甾体皂苷化合物，即(25S)-27-羟基-偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→4)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷{(25S)-27-hydroxy-penogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside, 1} 、(25S)-27- 羟基- 偏诺皂苷元-3β-O-α-L- 吡喃鼠李糖基-(1→4)-O-α-L-吡喃鼠李糖基-(1→4)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷(polyphylloside III, 2)，应用¹H NMR，¹³C NMR，DEPT，HSQC，HMBC，¹H-¹H COSY 和NOESY NMR 技术进行结构解析，并对化合物1，2 的¹H NMR，¹³C NMR 数据进行全归属．

关键词: 核磁共振(NMR), 2D NMR, 甾体皂苷, 全归属

Abstract:

(25S)-27-hydroxypenogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside (1) and polyphylloside III (2) were isolated from Trillium tschonoskii by column chromatography. One-dimensional and two-dimensional NMR techniques, including ¹H NMR, ¹³C NMR, DEPT, HSQC, HMBC and ¹H-¹H COSY, were used to elucidate the structures of these compounds. ¹³C NMR and ¹H NMR chemical shifts of the two compounds were completely assigned.

Key words: NMR, two-dimensional NMR, steroid glycosides, assignment

中图分类号:

O482.53

柴江1，王欣3，李慧敏4，张欣1，杨芳1，唐志书1，宋小妹1，裴月湖4，梅其炳2，岳正刚1,2. 延龄草中2 个甾体皂苷的2D NMR 研究[J]. 波谱学杂志, 2015, 32(1): 105-115.

CHAI Jiang1，WANG Xin3，LI Hui-min4，ZHANG Xin1，YANG Fang1，TANG Zhi-shu1，SONG Xiao-mei1，PEI Yue-hu4，MEI Qi-bing2，YUE Zheng-gang1,2. A Two-Dimensional NMR Study on Steroid Glycosides Extracted from Trillium tschonoskii[J]. Chinese Journal of Magnetic Resonance, 2015, 32(1): 105-115.

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延龄草中2 个甾体皂苷的2D NMR 研究

A Two-Dimensional NMR Study on Steroid Glycosides Extracted from Trillium tschonoskii

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