大鼠尿液中N-氧化烟酰胺和伪尿嘧啶核苷的NMR分析

波谱学杂志

大鼠尿液中N-氧化烟酰胺和伪尿嘧啶核苷的NMR分析

安艳捧^1,2，杨晓艳^1,2，李洪德^1,2，李宁^1,2，唐惠儒^1*

1. 中国科学院生物磁共振分析重点实验室，波谱与原子分子物理国家重点实验室，武汉磁共振中心（中国科学院武汉物理与数学研究所），湖北武汉 430071;2. 中国科学院大学，北京 100049

收稿日期:2013-05-20 修回日期:2013-07-21 出版日期:2014-06-05 发布日期:2014-06-05
作者简介:安艳捧(1983-)，男，山东青州人，博士研究生，主要从事基于核磁共振的代谢组学研究． *通讯联系人：唐惠儒，电话：027-87198430，E-mail：huiru.tang@wipm.ac.cn.
基金资助:
国家自然科学基金资助项目(21175149, 21221064)；国家重点基础研究发展计划资助项目(2010CB912501, 2012CB934004)．

NMR Analysis of Nicotinamide N-Oxide and Pseudouridine in Rat Urine

AN Yan-peng^1,2，YANG Xiao-yan^1,2，LI Hong-de^1,2，LI Ning^1,2，TANG Hui-ru^1*

1. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2013-05-20 Revised:2013-07-21 Online:2014-06-05 Published:2014-06-05
About author:*Corresponding author：TANG Hui-ru, Tel: 027-87198430，E-mail：huiru.tang@wipm.ac.cn.
Supported by:
国家自然科学基金资助项目(21175149, 21221064)；国家重点基础研究发展计划资助项目(2010CB912501, 2012CB934004)．

摘要/Abstract

摘要：

N-氧化烟酰胺和伪尿嘧啶核苷是尿液代谢组的两种重要组成成分，但是其核磁共振信号的归属仍不完善．该文采用多种2D NMR谱(¹H-¹H COSY，¹H-¹H TOCSY，¹H-¹H J-RES，¹H-¹³C HSQC和¹H-¹³C HMBC)对尿液中N-氧化烟酰胺和伪尿嘧啶核苷的结构进行了确定，并对其¹H和¹³C的化学位移进行了归属．

关键词: 液体核磁共振(liquid NMR), 归属, 二维核磁共振(2D NMR), N-氧化烟酰胺, 伪尿嘧啶核苷

Abstract:

Nicotinamide N-oxide and pseudouridine are two important metabolites in rat urine. In this studies, the ¹H and ¹³C NMR signals from these two metabolites were assigned using a series of two-dimensional (2D) NMR spectra including ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹H J-RES, ¹H-¹³C HSQC and ¹H-¹³C HMBC.

Key words: pseudouridine, liquid NMR, assignment, 2D NMR, nicotinamide N-oxide

中图分类号:

O482.53

安艳捧1,2，杨晓艳1,2，李洪德1,2，李宁1,2，唐惠儒1*. 大鼠尿液中N-氧化烟酰胺和伪尿嘧啶核苷的NMR分析[J]. 波谱学杂志.

AN Yan-peng1,2，YANG Xiao-yan1,2，LI Hong-de1,2，LI Ning1,2，TANG Hui-ru1* . NMR Analysis of Nicotinamide N-Oxide and Pseudouridine in Rat Urine[J]. Chinese Journal of Magnetic Resonance.

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