水溶性镧系位移试剂对羟基脯氨酸的手性识别

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (4): 521-529.

水溶性镧系位移试剂对羟基脯氨酸的手性识别

刘洋来，许美凤，颜贤忠^*

国家生物医学分析中心，北京 100850

收稿日期:2012-02-03 修回日期:2012-03-23 出版日期:2012-12-05 发布日期:2012-12-05
基金资助:
国家科技重大专项“重大新药创制”资助项目（2009ZX09301-002，2009ZX09304-004）.

Chiral Recognition of Hydroxyproline by Water-Soluble Chiral Lanthanide Shift Reagent

LIU Yang-Lai, XU Mei-Feng, YAN Xian-Zhong^*

National Center of Biomedical Analysis, Beijing 100850, China

Received:2012-02-03 Revised:2012-03-23 Online:2012-12-05 Published:2012-12-05
Supported by:
国家科技重大专项“重大新药创制”资助项目（2009ZX09301-002，2009ZX09304-004）.

摘要/Abstract

摘要：

用¹H NMR和¹³ C NMR研究了水溶性镧系位移试剂Sm-pdta对反式-4-羟基脯氨酸的手性识别. 反式-4-羟基脯氨酸与Sm-pdta形成络合物后，其¹H NMR和¹³C NMR图谱的化学位移有所变化，手性碳附近的某些原子所对应的吸收峰裂分为2组信号，分别对应反式-4-羟基脯氨酸的两种对映异构体. 结果显示，对于反式-4-羟基脯胺酸, Sm-pdta是一个既方便又有效的手性位移试剂.

关键词: 核磁共振(NMR), 反式-4-羟基脯胺酸, , Sm-pdta, 手性位移试剂, 手性识别

Abstract:

¹H and ¹³C NMR spectroscopy was carried out to investigate the chiral recognition of trans-4-hydroxyproline using water-soluble chiral lanthanide shift reagent Sm-pdta. When complexed with Smpdta, most signals in the ¹H and ¹³C NMR spectra of trans-4-hydroxy-L-proline and trans-4-hydroxy-D-proline were shifted, leading to separation of the signals from the two enantiomers. The results showed that Sm-pdta is an effective reagent for chiral recognition of trans-4-hydroxyproline.

Key words: NMR, trans-4-hydroxyproline, Sm-pdta, chiral shift reagent, chiral recognition

中图分类号:

O482.53

刘洋来, 许美凤, 颜贤忠. 水溶性镧系位移试剂对羟基脯氨酸的手性识别[J]. 波谱学杂志, 2012, 29(4): 521-529.

LIU Yang-Lai, XU Mei-Feng, YAN Xian-Zhong. Chiral Recognition of Hydroxyproline by Water-Soluble Chiral Lanthanide Shift Reagent[J]. Chinese Journal of Magnetic Resonance, 2012, 29(4): 521-529.

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