常规T2像和组织学研究APPswe/PS1dE9转基因小鼠模型

波谱学杂志 ›› 2011, Vol. 28 ›› Issue (1): 127-134.

常规T₂像和组织学研究APPswe/PS1dE9转基因小鼠模型

徐威^1*，湛颜强²

1. 波谱与原子分子物理国家重点实验室（中国科学院武汉物理与数学研究所），湖北武汉 430071； 
2. 华中科技大学同济医学院附属同济医院神经科，湖北武汉 430030

出版日期:2011-03-05 发布日期:2011-03-05
基金资助:
国家重点基础研究发展计划(“973”计划）资助项目(2006CB705600)，国家自然科学基金资助项目(30870674，0921004).

T₂-Weighted Imaging and Histological Assessment on a Transgenic APPswe/PS1dE9 Mouse Model

XU Wei^1*, ZHAN Ya-Qang²

1. State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Online:2011-03-05 Published:2011-03-05
Supported by:
国家重点基础研究发展计划(“973”计划）资助项目(2006CB705600)，国家自然科学基金资助项目(30870674，0921004).

摘要/Abstract

摘要：

用常规的T₂加权像研究阿尔茨海默病双转基因APPswe/PS1dE9小鼠模型，拟合了海马和顶叶皮层的T₂，并用组织学方法研究了APP/PS1小鼠在这2个感兴趣区域随年龄相关的淀粉样斑块病理的发展及铁质的沉积. T₂拟合的结果显示，较高月龄的APP/PS1小鼠相比于低月龄APP/PS1小鼠T₂有升高但不显著，各月龄组APP/PS1小鼠与同窝生非阳性小鼠之间也无明显差异；组织学的结果则显示年龄相关的淀粉样斑块的增多，铁质在16月龄的APP/PS1小鼠脑区中有明显沉积且和斑块具有“共域性”. 两方面的实验结果提示：在该模型淀粉样斑块沉积病理发展的早期，这2个脑区的T₂并不能作为特异性的病理发展的标志.

关键词: 磁共振成像(MRI), T₂加权像, 淀粉样斑块, 铁质沉积, 阿尔茨海默病

Abstract:

We studied a double transgenic APPSwe/PS1dE9 mouse model using T₂-weighted imaging and histological assessment to detect agerelated amyloidosis and iron load in the hippocampus and cortex. The results indicated that T₂ of older transgenic mice was slightly higher than that of the younger ones, but the difference did not reach statistical significance. We also did not found any significant T₂ difference between transgenic mice and age-matched non-transgenic littermates. Histological assessment revealed an age-related increase of amyloid load in the hippocampus and parietal cortex. Positive iron staining was observed in amyloid-rich regions of 16-month-old APP/PS1 mice, and co-localized, to a large extent, with amyloid deposits. These results indicated that T₂ might not be sensitive enough to detect amyloid and iorn deposition in this model.

Key words: MRI, T₂-weighted imaging, amyloid-β, iron load, Alzheimer’s disease

中图分类号:

R445.2

徐威, 湛颜强. 常规T₂像和组织学研究APPswe/PS1dE9转基因小鼠模型[J]. 波谱学杂志, 2011, 28(1): 127-134.

XU Wei, ZHAN Ya-Qang. T₂-Weighted Imaging and Histological Assessment on a Transgenic APPswe/PS1dE9 Mouse Model[J]. Chinese Journal of Magnetic Resonance, 2011, 28(1): 127-134.

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常规T₂像和组织学研究APPswe/PS1dE9转基因小鼠模型

T₂-Weighted Imaging and Histological Assessment on a Transgenic APPswe/PS1dE9 Mouse Model

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