固体核磁共振研究淀粉样蛋白纤维的进展

波谱学杂志

固体核磁共振研究淀粉样蛋白纤维的进展

张正逢^1,2，杨俊^1*

1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心(中国科学院武汉物理与数学研究所)，湖北武汉 430071； 
2. 中国科学院大学，北京 100049

收稿日期:2012-05-24 修回日期:2012-06-19 出版日期:2013-06-05 发布日期:2013-06-05
作者简介:张正逢(1985-)，男，江西泰和人,博士研究生,主要从事蛋白质的固体核磁共振研究. *通讯联系人：杨俊，电话：027-87199723，E-mail：yangjun@wipm.ac.cn.
基金资助:
国家自然科学基金资助项目(21075133).

Solid-State NMR Studies on Amyloid Fibrils: Recent Progresses

ZHANG Zheng-feng^1,2, YANG Jun^1*

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center of Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-05-24 Revised:2012-06-19 Online:2013-06-05 Published:2013-06-05
About author:张正逢(1985-)，男，江西泰和人,博士研究生,主要从事蛋白质的固体核磁共振研究. *通讯联系人：杨俊，电话：027-87199723，E-mail：yangjun@wipm.ac.cn.
Supported by:
国家自然科学基金资助项目(21075133).

摘要/Abstract

摘要：

淀粉样蛋白纤维是一类纤维状的蛋白质聚集体，与多种蛋白质沉积疾病相关. 对淀粉样蛋白纤维结构的研究，有助于人们从分子水平上阐述其形成机理，提供相关疾病预防或治疗的依据. 由于淀粉样蛋白纤维不可溶、非结晶，因此液体核磁共振和X-射线衍射等方法对这类体系的应用受限，而固体核磁共振被认为是研究这类体系最具前景的技术. 该综述介绍了固体核磁共振解析蛋白质结构的方法及其应用于淀粉样蛋白纤维体系的研究进展.

关键词: 固体核磁共振(solid-state NMR), 淀粉样蛋白纤维, 三维结构, 魔角旋转, 蛋白质, 二级结构

Abstract:

Amyloid fibrils are fibriform aggregates of amyloid proteins, which are implicated in many protein deposition diseases. Knowing the structures of amyloid fibrils will shed light on the molecular mechanism of fibrillation, facilitating the prevention and therapy of related diseases. Solid-state NMR is the most prospective technique for elucidating the structures of amyloid fibrils, since they are insoluble and non-crystalline such that they are difficult to be studied by liquid-state NMR and X-ray diffraction approaches. In this paper, recent progresses on using solid-state NMR methods to elucidate structural information of proteins, especially amyloid fibrils, are reviewed.

Key words: solid-state NMR, amyloid fibril, three-dimensional structure, magic angle spinning, protein, secondary structure

中图分类号:

O482.53

张正逢1,2，杨俊1*. 固体核磁共振研究淀粉样蛋白纤维的进展[J]. 波谱学杂志.

ZHANG Zheng-feng1,2, YANG Jun1*. Solid-State NMR Studies on Amyloid Fibrils: Recent Progresses[J]. Chinese Journal of Magnetic Resonance.

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