波谱学杂志 ›› 2010, Vol. 27 ›› Issue (1): 89-94.

• 恭祝徐广智先生八十华诞(专辑) • 上一篇    下一篇

细胞灌注-核磁共振联用方法的研究

  

  1. 1.贵州师范大学 化学与材料科学学院, 贵州 贵阳 550001;
    2.贵州省毕节学院 化学系, 贵州 毕节 551700;
    3.贵州大学 精细化工研究开发中心,贵州 贵阳 550025
  • 收稿日期:2009-12-30 出版日期:2010-03-05 发布日期:2010-03-05
  • 基金资助:

    国家自然科学基金资助项目(20765001);贵州省科技基金资助项目[黔科合J字(2006)2012].

A Cell Perfusion System for Online NMR Studies

  1. 1.Chemistry Department, Guizhou Normal University, Guiyang 550001, China;
    2.Chemistry Department, Guizhou Bijie College, Bijie 551700, China;
    3.Center for R & D of Fine Chemicals, Guizhou University, Guiyang 550025, China
  • Received:2009-12-30 Online:2010-03-05 Published:2010-03-05
  • Supported by:

    国家自然科学基金资助项目(20765001);贵州省科技基金资助项目[黔科合J字(2006)2012].

摘要:

 该文报道了研制的一套细胞灌注装置,并运用该细胞灌注装置与核磁共振连用的方法在线监测药物作用下肿瘤细胞的能量代谢过程.  结果表明核磁共振细胞灌注方法可有效观察到细胞能量代谢的变化,可作为追踪筛选药物的活性成分及探索药物治疗肿瘤细胞的作用机理的一种有力手段.

关键词: 核磁共振(NMR), 灌注培养, 31P NMR

Abstract:

A cell perfusion system for online NMR studies is reported. The system was used to measure 31P NMR signals of ATP in living breast cancer cells (MCF-7), with or without perfusion with cantharidin solution. The results- showed that online NMR on perfused cell culture is an effective way to identify active components from extracts of traditional Chinese medicine. The method can also be used to study the interactions between drugs and cancer cells.

Key words: NMR, cell perfusion, 31P NMR

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