电离辐射对小鼠肾脏影响的代谢组学研究

doi:10.11938/cjmr20182622

波谱学杂志 ›› 2019, Vol. 36 ›› Issue (2): 172-181.doi: 10.11938/cjmr20182622

电离辐射对小鼠肾脏影响的代谢组学研究

肖雄杰^1,2,3, HU Mary³, 张许¹, HU Jian-zhi³

1. 波谱与原子分子物理国家重点实验室, 武汉磁共振中心(中国科学院武汉物理与数学研究所), 湖北武汉 430071;
2. 中国科学院大学, 北京 100049;
3. Pacific Northwest National Laboratory, Richland, WA 99352

收稿日期:2018-03-31 发布日期:2018-04-21
通讯作者: 张许 Tel:+86-027-87197056, E-mail:zhangxu@wipm.ac.cn;HU Jian-zhi Tel:+1-5093716544, E-mail:Jianzhi.Hu@pnnl.gov E-mail:zhangxu@wipm.ac.cn;Jianzhi.Hu@pnnl.gov
基金资助:
the National Science Foundation of China (21505153, 21120102038, 21675170, 81227902, 21475146, 21221064); Key Research Program of Frontier Sciences, CAS (QYZDJ-SSW-SLH027); the Award from National Institute of Environmental Health Sciences of the National Institute of Health (NIH) (R01ES022176).

An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation

XIAO Xiong-jie^1,2,3, HU Mary³, ZHANG Xu¹, HU Jian-zhi³

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan(Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Pacific Northwest National Laboratory, Richland, WA 99352, USA

Received:2018-03-31 Published:2018-04-21
Supported by:
the National Science Foundation of China (21505153, 21120102038, 21675170, 81227902, 21475146, 21221064); Key Research Program of Frontier Sciences, CAS (QYZDJ-SSW-SLH027); the Award from National Institute of Environmental Health Sciences of the National Institute of Health (NIH) (R01ES022176).

摘要/Abstract

摘要： 电离辐射对生物体影响是目前生物医学领域研究的热点之一.本文利用基于核磁共振氢谱（¹H NMR）的代谢组学技术研究来自暴露于不同的电离辐射的C57BL/6J小鼠的肾脏的水溶性萃取物，并试图寻找电离辐射对小鼠肾脏影响的特异性生物标志物.分析结果表明，辐射组可与相应的对照组明显区分.在所发现的75个代谢物中，有6种代谢物的浓度发生显著性变化，分别为2-氨基丁酸、3-羟基丁酸、高丝氨酸、1，3-丙二胺、β-丙氨酸和抗坏血酸.这些代谢物可能成为高剂量电离辐射对生物体肾脏代谢影响的标志物.

关键词: 核磁共振氢谱(¹H NMR), 代谢组学, 电离辐射, 肾脏, 多变量数据分析

Abstract: The biological effects of ionizing radiation on organisms have emerged as a hot topic for research. In this study, the changes of metabolic profile in the kidney tissues extracts from C57BL/6J mice with or without exposing to whole body gamma or proton radiation were investigated with the liquid state proton nuclear magnetic resonance (¹H NMR)-based metabolomics approach. The results demonstrated that the exposed group had significantly different kidney metabolic profiles from the non-exposed group, involving mainly six metabolites, including 2-aminobutyrate, 3-hydroxybutyrate, homoserine, 1,3-diaminopropane, β-alanine and ascorbate. These metabolites could be used as potential biomarkers for the biological effects of high-dose ionizing radiation on kidney metabolism.

Key words: proton nuclear magnetic resonance (¹H NMR), metabolomics, ionizing radiation, kidney, multivariate data analysis

中图分类号:

O482.53

肖雄杰, HU Mary, 张许, HU Jian-zhi. 电离辐射对小鼠肾脏影响的代谢组学研究[J]. 波谱学杂志, 2019, 36(2): 172-181.

XIAO Xiong-jie, HU Mary, ZHANG Xu, HU Jian-zhi. An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 172-181.

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电离辐射对小鼠肾脏影响的代谢组学研究

An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation

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