基于 1H NMR谱的给药赭石大鼠血清代谢组学研究

doi:10.11938/cjmr20150408

波谱学杂志 ›› 2015, Vol. 32 ›› Issue (4): 628-636.doi: 10.11938/cjmr20150408

基于 ¹H NMR谱的给药赭石大鼠血清代谢组学研究

魏莹^1,2，章文军¹，薛蓉²，李晓晶²*，裴奉奎²

1. 河北工业大学化工学院，天津 300130；
2. 中国科学院长春应用化学研究所，吉林长春 130022

收稿日期:2014-12-04 修回日期:2015-10-23 出版日期:2015-12-05 发布日期:2015-12-05
作者简介:魏莹(1989-)，女，河北唐山人，硕士研究生，应用化学专业. *通讯联系人：李晓晶，电话： 0431-85262219，E-mail： xjli@ciac.ac.cn.
基金资助:
国家自然科学基金资助项目(20975097, 21305134)

A ¹H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats

WEI Ying^1,2， ZHANG Wen-jun¹， XUE Rong²， LI Xiao-jing²*， PEI Feng-kui²

1. Department of Chemistry, Hebei University of Technology, Tianjin 300130, China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2014-12-04 Revised:2015-10-23 Online:2015-12-05 Published:2015-12-05
About author:*Corresponding author： LI Xiao-jing, Tel: +86-431-85262219, E-mail: xjli@ciac.ac.cn.
Supported by:
国家自然科学基金资助项目(20975097, 21305134)

摘要/Abstract

摘要：

基于¹H NMR 的代谢组学方法对灌胃给药赭石的成年 Wistar 大鼠血清进行分析，运用主成分分析模式识别方法对所得数据进行处理，并对给药大鼠血清进行生化指标检测．研究结果表明，大鼠体内β-羟异丁酸、乙酸、丙酮、胆碱、甘油磷脂酰胆碱、葡萄糖、乳酸、低密度脂蛋白、极低密度脂蛋白和脂质等内源性代谢物浓度发生明显变化，可作为赭石的特征代谢物． 2 g/kg 和 5 g/kg 体重剂量赭石使大鼠机体产生大量活性氧化物(ROS)，造成过氧化损伤，导致能量代谢和糖代谢紊乱，糖酵解反应增强，并且对肝功能造成了影响．

关键词: 核磁共振(NMR), 代谢组学, 主成分分析, 赭石

Abstract:

Wistar rats were intragastrically administered with different doses (0, 2, 5 g/kg body weight) of haematitum. ¹H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of β-hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species (ROS) and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.

Key words: nuclear magnetic resonance, metabonomics, principal component analysis, haematitum

中图分类号:

O482.53

魏莹1,2，章文军1，薛蓉2，李晓晶2*，裴奉奎2. 基于 ¹H NMR谱的给药赭石大鼠血清代谢组学研究
[J]. 波谱学杂志, 2015, 32(4): 628-636.

WEI Ying1,2， ZHANG Wen-jun1， XUE Rong2， LI Xiao-jing2*， PEI Feng-kui2. A ¹H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats[J]. Chinese Journal of Magnetic Resonance, 2015, 32(4): 628-636.

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基于 ¹H NMR谱的给药赭石大鼠血清代谢组学研究

A ¹H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats

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