乙酰甲喹影响猪尿液代谢组的NMR研究

doi:10.11938/cjmr20160305

波谱学杂志 ›› 2016, Vol. 33 ›› Issue (3): 406-414.doi: 10.11938/cjmr20160305

乙酰甲喹影响猪尿液代谢组的NMR研究

万钱芬^1,3, 何庆华², 王玉兰¹

1. 波谱与原子分子物理国家重点实验室, 武汉磁共振中心(中国科学院武汉物理与数学研究所), 湖北武汉 430071;
2. 深圳大学化学与环境工程学院, 深圳 518060;
3. 中国科学院大学, 北京 100049

收稿日期:2015-11-06 修回日期:2016-07-11 出版日期:2016-09-05 发布日期:2016-09-05
通讯作者: 王玉兰,电话:027-87198430,E-mail:yulan.wang@wipm.ac.cn. E-mail:yulan.wang@wipm.ac.cn
作者简介:万钱芬(1988-),女,四川广安人,博士研究生,主要从事核磁共振的代谢组学研究,E-mail:wanqianfen1988@126.com.
基金资助:
国家重点基础研究发展计划（“973”计划）资助项目（2009CB118804）.

Effects of Acute Mequindox Exposure on Urinary Metabonome in Pigs

WAN Qian-fen^1,3, HE Qing-hua², WANG Yu-lan¹

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2015-11-06 Revised:2016-07-11 Online:2016-09-05 Published:2016-09-05

摘要/Abstract

摘要：

乙酰甲喹是喹噁啉类广谱抗菌药，在我国畜禽养殖业中有广泛的应用. 乙酰甲喹的毒性以及毒性机制被越来越多的报道，然而，乙酰甲喹对动物内源性代谢的影响仍不清楚. 该研究利用基于核磁共振（NMR）的代谢组学方法并结合多变量数据分析方法研究了乙酰甲喹一次性急性给药（10 mg/kg.bw）对猪尿液代谢组的影响. 结果发现，乙酰甲喹给药后，尿液中肌酸、马尿酸和对甲酚葡萄糖醛酸浓度显著降低，而对甲基苯酚和苯甲酸的浓度在给药后显著升高. 乙酰甲喹对猪尿液代谢组的影响在给药144 h后未见恢复. 研究结果显示，剂量为10 mg/kg.bw乙酰甲喹给药主要影响了猪体内肠道菌群微生态. 上述研究为乙酰甲喹对生物体内源性代谢的影响提供了基础数据，并为乙酰甲喹的安全用药提供了数据参考.

关键词: 核磁共振(NMR), 肠道菌群, 尿液代谢组, 乙酰甲喹

Abstract:

Mequindox is a member of the quinoxaline family, and widely used in Chinese veterinary industry as an antibacterial drug. Toxicity of mequindox and the underlying mechanisms have been studied previously. However, endogenous metabolic alterations in animals caused by mequindox exposure are currently unknown. This study investigated the effects of acute mequindox exposure (10 mg/kg body weight) on urinary metabonome in pigs using an NMR-based metabonomics approach. The results showed that acute mequindox exposure significantly decreased the levels of creatine, hippurate and p-cresol glucuronide in the urine of treated pigs, whereas the levels of p-cresol and benzoate increased significantly. The changes of urinary metabolic profiles were long-lasting, and did not recover at 144 h post-exposure. At the dose given, mequindox mainly caused disturbance of gut microbial ecosystem. This study provided useful information on the effects of mequindox on metabolism in pigs, and might guide the safe application of mequindox in veterinary industry.

Key words: NMR, gut microbes, urinary metabonome, mequindox

中图分类号:

O482.53

万钱芬, 何庆华, 王玉兰. 乙酰甲喹影响猪尿液代谢组的NMR研究[J]. 波谱学杂志, 2016, 33(3): 406-414.

WAN Qian-fen, HE Qing-hua, WANG Yu-lan. Effects of Acute Mequindox Exposure on Urinary Metabonome in Pigs[J]. Chinese Journal of Magnetic Resonance, 2016, 33(3): 406-414.

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乙酰甲喹影响猪尿液代谢组的NMR研究

Effects of Acute Mequindox Exposure on Urinary Metabonome in Pigs

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