小鼠甲醇氧化应激的电子顺磁共振的研究

波谱学杂志

小鼠甲醇氧化应激的电子顺磁共振的研究

周丽芳1，朱翔1，赵红莉1，蓝闽波1,2*, 史新梅2

1. 华东理工大学，上海市功能性材料化学重点实验室，上海 200237； 
2. 华东理工大学，分析测试中心，上海 200237

收稿日期:2013-01-25 修回日期:2013-03-05 出版日期:2013-12-05 发布日期:2013-12-05
作者简介:周丽芳（1975-），女，江苏，博士研究生，工程师，主要从事磁共振研究.*通讯联系人：蓝闽波，电话：021-64253574,E- mail: minbolan@ecust.edu.cn.
基金资助:
上海市科委纳米专项资助项目（12nm0501100）.

Methanol-Induced Oxidative Stress in Mice Studied by Electron Paramagnetic Resonance

ZHOU Li-fang1，ZHU Xiang1，ZHAO Hong-li1，LAN Min-bo1,2*，SHI Xin-mei2

1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China; 
2. Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, China

Received:2013-01-25 Revised:2013-03-05 Online:2013-12-05 Published:2013-12-05
About author:*Corresponding author: Lan Min-bo, Tel: 021-64253574, E-mail: minbolan@ecust.edu.cn.
Supported by:
上海市科委纳米专项资助项目（12nm0501100）.

摘要/Abstract

摘要：

建立了小鼠甲醇中毒的氧化应激模型，研究了甲醇诱导小鼠产生氧化应激状态，探讨了大黄中草药和维生素C对小鼠甲醇氧化应激的保护作用. 模型小鼠共分为5个组：空白组、对照组、甲醇应激组、大黄组、维生素C（Vc）组. 利用电子自旋捕捉技术研究了小鼠甲醇氧化应激不同模型组小鼠体内的肝、肾、脾、心、肺和脑中产生的自由基强度的变化. 结果表明：甲醇诱发了小鼠体内自由基的产生，甲醇组和生理盐水组相比，自由基的强度明显增强（显著性差异统计指标P<0.01)；而大黄和Vc对小鼠甲醇氧化应激有保护作用，大黄组、Vc 组和甲醇组相比自由基的强度则明显降低（P<0.01).

关键词: 电子顺磁共振(EPR), 氧化应激, 自旋捕捉, 小鼠, 甲醇, 自由基

Abstract:

A mouse model of acute methanol poisoning was established to evaluate the protective effects of rhubarb and vitamin C (Vc) on methanol-induced oxidative stress in different organs. The mice used in the study were randomly divided into five group: blank group, control group, methanol group, rhubarb group and Vc group. EPR spin trapping technique was employed to study the intensity change of spin adducts induced by reactive oxygen species (ROS) free radicals generated in different organs of the mice. Compared with the control group, the animals in the methanol group showed significantly increased amount of ROS free radicals in the organs, suggesting the induction of acute oxidative stress. Rhubarb and Vc were shown to be able to alleviate acute methanolinduced oxidative stress.

Key words: EPR, oxidative stress, spin trapping, mice, methanol, free radical

中图分类号:

O657.61

周丽芳1，朱翔1，赵红莉1，蓝闽波1,2*, 史新梅2. 小鼠甲醇氧化应激的电子顺磁共振的研究[J]. 波谱学杂志.

ZHOU Li-fang1，ZHU Xiang1，ZHAO Hong-li1，LAN Min-bo1,2*，SHI Xin-mei2. Methanol-Induced Oxidative Stress in Mice Studied by Electron Paramagnetic Resonance[J]. Chinese Journal of Magnetic Resonance.

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