叶酸偶联磁性白蛋白纳米球的体内外靶向性评价

波谱学杂志

叶酸偶联磁性白蛋白纳米球的体内外靶向性评价

唐秋莎1*，安艳丽2，杨蕊1

1. 东南大学医学院病理与病理生理学系，江苏南京 210009； 
2. 东南大学医学院，江苏省分子影像与功能影像重点实验室，江苏南京 210009

收稿日期:2013-06-18 修回日期:2013-07-16 出版日期:2013-12-05 发布日期:2013-12-05
作者简介:唐秋莎（1971-），女，江苏南京人，博士，副教授，影像医学与核医学专业. 电话：025-83272373，E-mail: panyixi-tqs@163.com. *通讯联系人.
基金资助:
国家自然科学基金资助项目(81071881).

In Vitro and In Vivo Evaluation of Tumor-Targeting Efficacy of Folate-Conjugated Magnetic Albumin Nanospheres by MRI

TANG Qiu-sha1*，AN Yan-li2，YANG Rui1

1. Department of Pathology and Pathophysiology, Southeast University, Nanjing 210009, China; 
2. Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Medical School of Southeast University, Nanjing 210009, China

Received:2013-06-18 Revised:2013-07-16 Online:2013-12-05 Published:2013-12-05
About author:*Corresponding author: Tang Qiu-sha,Tel: 025-83272373，E-mail: panyixi-tqs@163.com.
Supported by:
国家自然科学基金资助项目(81071881).

摘要/Abstract

摘要：

该文从体内外水平，探讨叶酸偶联磁性白蛋白纳米球对人鼻咽癌细胞KB的靶向效应. 体外实验中，将叶酸靶向、非叶酸靶向和叶酸抑制组与人鼻咽癌细胞KB共孵育24 h后，通过普鲁士蓝染色、7.0 Tesla Micro-MR仪检测叶酸受体介导的靶向效应；体内试验中，首先建立裸鼠荷人鼻咽癌细胞KB皮下移植瘤动物模型，再通过MRI检测及病理组织学检查，评价叶酸偶联磁性白蛋白纳米球的靶向性. 体外MRI成像显示叶酸靶向组与人鼻咽癌细胞KB共孵育后T₂WI信号强度降低，普鲁士蓝染色则显示靶向组细胞内存在大量铁颗粒；在体MRI成像结果显示，注射材料后不同时间点叶酸靶向组的T₂WI、T*₂-WI强度有明显下降，具有统计学差异. 病理学检查显示叶酸靶向组肿瘤组织内有较多蓝染的铁颗粒，而非靶向组和叶酸抑制组则未见铁颗粒的存在. 体内外实验均表明叶酸偶联磁性白蛋白纳米球对人鼻咽癌细胞KB具有良好的靶向性.

关键词: 磁共振成像(MRI), 靶向成像, 叶酸, 癌

Abstract:

This study evaluated the tumor-targeting efficacy of folate-conjugated magnetic albumin nanospheres with in vitro and in vivo MRI experiments. Human nasopharyngeal epidermal KB cells were used for the in vitro experiment, which were incubated with folate-conjugated and non-folate-conjugated nanospheres for 24 hours. The presence of the nanospheres inside the cells were then detected using MRI and Prussian blue staining. For the in vivo experiment, nude mice were transplanted subcutaneously with nasopharyngeal epidermal KB tumor. MRI and histopathological examination were used to detect the presence of nanospheres in the tumors after injection. The in vitro and in vivo experiments demonstrated that folate-conjugated nanospheres had significantly higher amount of deposition in the tumor cells/tumors than the non-folate-conjugated nanospheres. It was concluded that folate-conjugated magnetic albumin nanospheres had a better targeting efficacy on human nasopharyngeal epidermal KB cells, relative to the non-folate-conjugated nanospheres.

Key words: magnetic resonance imaging, targeting imaging, folate, cancer

中图分类号:

O482.53

唐秋莎1*，安艳丽2，杨蕊1. 叶酸偶联磁性白蛋白纳米球的体内外靶向性评价[J]. 波谱学杂志.

TANG Qiu-sha1*，AN Yan-li2，YANG Rui1. In Vitro and In Vivo Evaluation of Tumor-Targeting Efficacy of Folate-Conjugated Magnetic Albumin Nanospheres by MRI[J]. Chinese Journal of Magnetic Resonance.

参考文献

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