不同生长期当归 1H NMR指纹图谱的研究

波谱学杂志

不同生长期当归 ¹H NMR指纹图谱的研究

杨应文¹，王亚丽^1*，萨日娜¹，潘新波¹，肖宇奇¹，孙宇靖¹，王兰霞²

1. 甘肃中医学院甘肃省高校中(藏)药化学与质量研究省级重点实验室，当归研究所，甘肃兰州 730000； 
2 .甘肃省食品药品检验所, 甘肃兰州 730000

收稿日期:2012-01-13 修回日期:2012-09-03 出版日期:2013-03-05 发布日期:2013-03-05
通讯作者: 王亚丽,电话: 0931-8765470, E-mail: cnwyl1166@hotmail.com. E-mail:cnwyl1166@hotmail.com
作者简介:杨应文(1985-),男,甘肃礼县人,硕士研究生,主要从事中药质量控制的研究.电话: 13893128485,E-mail: yangying.wen2008@163.com.
基金资助:
国家自然科学基金资助项目(30960037), 甘肃省科技支撑计划资助项目(0804NKCA117), 甘肃省发改委战略性新兴产业和产业技术研究与开发专项项目.

Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by ¹H NMR

YANG Ying-wen¹, WANG Ya-li^1*, SA Ri-na¹, PAN Xin-bo¹, XIAO Yu-qi¹, SUN Yu-jing¹, WANG Lan-xia²

1. Provincial Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines, The Research Institute of Angelica Sinensis, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;
2. Gansu Institute for Food and Drug Control, Lanzhou 730000, China

Received:2012-01-13 Revised:2012-09-03 Online:2013-03-05 Published:2013-03-05
Contact: Wang Ya-li, Tel: 0931-8765470, E-mail: cnwyl1166@hotmail.com. E-mail:cnwyl1166@hotmail.com
About author:杨应文(1985-),男,甘肃礼县人,硕士研究生,主要从事中药质量控制的研究.电话: 13893128485,E-mail: yangying.wen2008@163.com.
Supported by:
国家自然科学基金资助项目(30960037), 甘肃省科技支撑计划资助项目(0804NKCA117), 甘肃省发改委战略性新兴产业和产业技术研究与开发专项项目.

摘要/Abstract

摘要：

利用¹H NMR法测定27个不同生长期的当归提取物，对当归的主要活性成分阿魏酸、藁本内酯、当归多糖进行了初步归属，用特征峰相对峰面积表示3种活性成分的相对含量，并对它们在生长过程中的含量变化进行分析. 数据分析显示，当归中阿魏酸、藁本内酯、当归多糖的含量在第1年变化较为平稳，第2年和第3年变化相对较为活跃，而在当归的生长周期内，当归多糖的含量变化明显大于其他两种活性成分，为参与代谢活动的主要活性成分. 结果证实，通过对照¹H NMR指纹图谱，将相对含量法与主成分分析法相结合，能对不同生长期的当归药材中的活性成分进行宏观评价和分析，可作为植物药材代谢组学的研究方法.

关键词: ¹H NMR指纹图谱, 含量变化, 当归, 生长期, 相对含量法, 主成分分析

Abstract:

The chemical fingerprints of 27 Angelica sinensis samples harvested at different growth stages were analyzed by ¹H NMR. Ferulic acid, ligustilide and angelica polysaccharide, three of the active constituents of Angelica sinensis, were identified, with their ¹H chemical shifts assigned. The relative peak areas of characteristic peaks were used to descript the changes of chemical contents in Angelica sinensis at different growth stages. The results showed that the levels of three constituents had little changes during the first year. More changes were observed in year two and year three. During the growth cycle of Angelica sinensis, the changes of angelica polysaccharide were greater than those of the other two constituents, which were proven to be the active ingredients involved in metabolism. It is concluded that ¹H NMR fingerprinting and principle component analysis can be used to analyze the active ingredient of Angelica sinensis at different growth periods.

Key words: ¹H NMR, chemical fingerprinting, Angelica sinensis, growth stage, principal component analysis

中图分类号:

O482.53

杨应文，王亚丽*，萨日娜，潘新波，肖宇奇，孙宇靖，王兰霞 . 不同生长期当归 ¹H NMR指纹图谱的研究[J]. 波谱学杂志.

YANG Ying-wen, WANG Ya-li*, SA Ri-na, PAN Xin-bo, XIAO Yu-qi, SUN Yu-jing, WANG Lan-xia. Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by ¹H NMR[J]. Chinese Journal of Magnetic Resonance.

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不同生长期当归 ¹H NMR指纹图谱的研究

Chemical Fingerprinting of Angelica Sinensis at Different Growth Stages by ¹H NMR

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