用异核多维NMR技术研究蛋白质动力学

摘要/Abstract

摘要：

蛋白质在溶液中的三维空间结构、动力学与蛋白质生物功能的关系是在分子水平上理解生命现象的重要基础. NMR技术在研究蛋白质动力学方面具有独特的优势，所能表征的运动过程相关时间尺度很广. 文章综述了异核多维NMR技术研究蛋白质动力学的实验技术和理论方法，介绍了描述蛋白质动力学的内运动参量的意义和Model-Free 方法，并举例说明¹⁵N弛豫测量实验被用于研究蛋白质及其与配体复合物的动力学. 

关键词: 异核多维核磁共振, 蛋白质, 动力学, 内运动, 构象交换

Abstract:

One of the fundamental problems in understanding life science at the molecular level is the relationship among structure, dynamics, and function of proteins. NMR has a unique capacity to investigate dynamics properties of proteins over a range of different time scales with atomic resolution. This review focuses on the experimental and theoretical methods used in heteronuclear spin relaxation measurement for studying protein dynamics, and the parameters describing the protein internal motion as well as the Model-Free method. Finally, some application examples are given in which ¹⁵N relaxation measurements were performed to study the backbone dynamics of protein and protein-ligand complex.

Key words: protein, relaxation, dynamics, mobility, heteronuclear NMR

中图分类号:

O482.53

廖新丽, 林东海. 用异核多维NMR技术研究蛋白质动力学[J]. 波谱学杂志, 2004, 21(4): 385-396.

LIAO Xin-Li, Lin-Dong-Hai. PROTEIN DYNAMICS STUDIED BY HETERONUCLEAR MULTI-DIMENSIONAL NMR[J]. Chinese Journal of Magnetic Resonance, 2004, 21(4): 385-396.

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