新型Gd基T2造影剂的制备和应用

doi:10.11938/cjmr20172558

波谱学杂志 ›› 2017, Vol. 34 ›› Issue (3): 302-310.doi: 10.11938/cjmr20172558

新型Gd基T₂造影剂的制备和应用

张艳辉^1,2, 张宏岩², 张海禄², 张朋利^1,2, 蒋海珍¹, 邓宗武², 谭波²

1. 上海大学理学院, 上海 200444;
2. 中国科学院苏州纳米技术与纳米仿生研究所, 江苏苏州 215123

收稿日期:2017-02-04 修回日期:2017-07-21 出版日期:2017-09-05 发布日期:2017-09-05
通讯作者: 谭波,Tel:0512-62872631,E-mail:btan2012@sinano.ac.cn. E-mail:btan2012@sinano.ac.cn
基金资助:
国家自然科学基金资助项目（31371010）.

A New Gd-Based T₂-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging

ZHANG Yan-hui^1,2, ZHANG Hong-yan², ZHANG Hai-lu², ZHANG Peng-li^1,2, JIANG Hai-zhen¹, DENG Zong-wu², TAN Bo²

1. College of Sciences, Shanghai University, Shanghai 200444, China;
2. Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China

Received:2017-02-04 Revised:2017-07-21 Online:2017-09-05 Published:2017-09-05

摘要/Abstract

摘要： 设计并合成了结构为TPP-Lys（Acp-DOTA-Gd）-COOH（简称Gd-DOTA-TPP）的小分子磁共振探针，通过电转染的方式用探针标记人源脐带间充质干细胞（hMSCs）.11.7 T磁共振成像（MRI）扫描结果表明，Gd-DOTA-TPP标记的hMSCs在细胞内Gd含量为9×10⁹ Gd/cell时，T₂加权信号强度即可低至背景信号强度，呈现较强暗信号.将Gd-DOTA-TPP标记的hMSCs移植入小鼠脑室，可明显提高移植干细胞在MRI设备上的检测灵敏度，检测限可低至10³个细胞.

关键词: 造影剂, 人源脐带间充质干细胞(hMSCs), 磁共振成像(MRI), 灵敏度, TPP-Lys (Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP)

Abstract: We synthesized TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP) as a novel T₂-weighted MRI contrast agent, and used it to label human mesenchymal stem cells (hMSCs) via electroporation. hMSCs labeled with Gd-DOTA-TPP presented a rather dark T₂-weighted image at a cellular Gd content of about 9×10⁹ Gd/cell. The labeled cells were syringe-injected into a mouse brain with defined cell numbers followed by T₂-weighted MRI on an 11.7 T system, which yield an in vivo imaging sensitivity of about 10³ cells.

Key words: contrast agent, human mesenchymal stem cells (hMSCs), magnetic resonance imaging (MRI), TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP), sensitivity

中图分类号:

O482.53

张艳辉, 张宏岩, 张海禄, 张朋利, 蒋海珍, 邓宗武, 谭波. 新型Gd基T₂造影剂的制备和应用[J]. 波谱学杂志, 2017, 34(3): 302-310.

ZHANG Yan-hui, ZHANG Hong-yan, ZHANG Hai-lu, ZHANG Peng-li, JIANG Hai-zhen, DENG Zong-wu, TAN Bo. A New Gd-Based T₂-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging[J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 302-310.

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新型Gd基T₂造影剂的制备和应用

A New Gd-Based T₂-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging

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