泽泻盐制前后成分转化的1H NMR分析

doi:10.11938/cjmr20160111

波谱学杂志 ›› 2016, Vol. 33 ›› Issue (1): 117-124.doi: 10.11938/cjmr20160111

泽泻盐制前后成分转化的¹H NMR分析

韩伟健, 林晓彤, 郭娜, 陈俏, 张婷婷, 许枬

辽宁中医药大学药学院, 大连 116600

收稿日期:2015-03-31 修回日期:2016-01-21 出版日期:2016-03-05 发布日期:2016-03-05
通讯作者: 许枬,电话:0411-85890191,E-mail:xudanbs@163.com. E-mail:xudanbs@163.com
作者简介:韩伟健(1991-),男,硕士研究生,研究方向为天然化学成分核磁共振谱学特征的研究,电话:0411-85890191,E-mail:youhwj@foxmail.com.
基金资助:
国家公益性行业专项课题资助项目(200807039).

Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by ¹H NMR Analysis

HAN Wei-jian, LIN Xiao-tong, GUO Na, CHEN Qiao, ZHANG Ting-ting, XU Nan

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China

Received:2015-03-31 Revised:2016-01-21 Online:2016-03-05 Published:2016-03-05

摘要/Abstract

摘要：

泽泻为临床常用中药,是五苓散、龙胆泻肝丸及六味地黄丸等经典方剂的主要组成.我国药典收载了泽泻的两种饮片,即生饮片和盐制片.传统理论认为泽泻生饮片和盐制片功效不同,生饮片偏重于利水泄热,盐制后泄热作用缓和,小剂量可用于补益剂,但其物质基础还不清楚,难以有效控制饮片的制备工艺和成品质量.为了深入揭示泽泻生饮片和盐制片的成分差异,以及盐制过程中成分的转化,该研究运用基于核磁共振氢谱(¹H NMR)技术的代谢组学方法研究了泽泻生饮片与盐制片的化学成分差异及盐制过程中的成分变化.结果表明,泽泻生饮片与盐制片成分差异较大,而且随着盐制时间的延长成分变化愈加明显.采用偏最小二乘法判别分析(PLS-DA)显示,δ_H 1.34信号对泽泻生饮片和盐制饮片在主成分1(t [1])方向的分离贡献最大.不同盐制时间条件下δ_H 3.82、3.78、3.66、1.34、0.92和1.30处的泽泻醇类成分特征信号变化明显,提示泽泻盐制过程中原萜烷型泽泻醇类成分可能发生脱水、脱乙酰基和氧化反应.该文为深入了解泽泻盐制过程成分转化提供了可靠依据.

关键词: 核磁共振氢谱(¹H NMR), 泽泻, 代谢组学, 炮制, 成分转化

Abstract:

As a commonly used traditional Chinese medicine (TMC), Alismatis rhizoma is the main ingredient of many classical formulas, such as Wu Ling San, Long Dan Xie Gan Wan and Liu Wei Di Huang Wan. Both the raw slices of Alismatis rhizoma and the slices processed with saline arelisted are prescription drugs in the Pharmacopoeia of People's Republic of China. TMC theory thinks the raw and processed slices of Alismatis rhizoma are different in function. The raw slices are emphasized on its actions of alleviating water retention and dispersing heat, whiles the processed slices are thought ineffective in these respects. The active components in these two types of Alismatis rhizoma slices remained poorly understood, making standardizing the saline processing procedure and quality control difficult. In this paper, we applied ¹H NMR based metabonomic approach to investigate the difference of chemical constitution between the raw and salt-processed slices of Alismatis rhizome. The result showed that the chemical constitution of the Alismatis rhizoma slices changed significantly after salt-processing, and such changes were influenced by the length of processing time. In PLS-DA analysis, the signals of alisols at δ 3.82, 3.78, 3.66, 1.34, 0.94, and 1.30 changed significantly under different processing conditions, indicating that alisols were perhaps the chemical fingreprints contributing the different functions of raw and salt-processed Alismatis rhizoma slices.

Key words: ¹H NMR, Alismatis rhizoma, metabonomic analysis, processing, chemical constitution

中图分类号:

O482.53

韩伟健, 林晓彤, 郭娜, 陈俏, 张婷婷, 许枬. 泽泻盐制前后成分转化的¹H NMR分析[J]. 波谱学杂志, 2016, 33(1): 117-124.

HAN Wei-jian, LIN Xiao-tong, GUO Na, CHEN Qiao, ZHANG Ting-ting, XU Nan. Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by ¹H NMR Analysis[J]. Chinese Journal of Magnetic Resonance, 2016, 33(1): 117-124.

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泽泻盐制前后成分转化的¹H NMR分析

Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by ¹H NMR Analysis

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