波谱学杂志 ›› 2001, Vol. 18 ›› Issue (4): 329-341.

• 研究论文 • 上一篇    下一篇

二维核磁共振波谱在阐明一种三萜多糖皂苷结构中的应用

鞠建华,周亮,杨峻山   

  1. 中国协和医科大学,中国医学科学院药用植物研究所,北京 100094
  • 收稿日期:2001-07-18 修回日期:2001-08-17 出版日期:2001-12-05 发布日期:2001-12-05
  • 作者简介:鞠建华(1972-,男,博士,助理研究员.
  • 基金资助:

    国家自然科学基金重点资助项目(No.29732040).

APPLICATION OF 2D NMR TECHNIQUES IN THE STRUCTURE ELUCIDATION OF AN OLEANOLIC TRITERPENIC GLYCOSIDE

JU Jian-hua, ZHOU Liang, YANG Jun-shan   

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094
  • Received:2001-07-18 Revised:2001-08-17 Online:2001-12-05 Published:2001-12-05
  • Supported by:

    国家自然科学基金重点资助项目(No.29732040).

摘要:

从毛茛科铁破锣属(Beesia)铁破锣[Beesia calthaefolia (Maxim.) Ulbr.]根茎的 正丁醇萃取物中分离得到一种齐墩果酸型三萜皂苷(1); 其化学结构通过化学方法和波谱分析(IR, ESI-MS, 1H NMR, 13C NMR, 1H-1H COSY, 1H-1H TOCSY, HMQC, HMQC-TOCSY, HMB C) 鉴定为齐墩果酸-3-O-β-D-吡喃葡萄糖(1→3)-α-L吡喃鼠李糖(1→2)-α -L-吡喃阿拉伯糖-28-O-α-L-吡喃鼠李糖(1→4)-β-D-吡喃葡萄糖(1→6)-β- D-吡喃葡萄糖酯苷. 本文具体介绍上述几种二维核磁共振技术在解析这一结构复杂的天然产物中的应用.

关键词: 铁破锣, 二维核磁共振, 波谱解析, 齐墩果酸型三萜皂苷

Abstract:

The rhizomes of \%Beesia calthaefolia\% yielded a triterpenic hexaglycoside  (1), the  structure of which was characterized by chemical and spectral means  (IR, ESI-MS, 1H NMR, 13C NMR, 1H-1H COSY, 1H -1H TOCSY, HMQC, HMQC-TOCSY, HMBC) as oleanolic acid-3-O-β-D-gluco pyranosyl (1-3)-α-L-rhamnopyranosyl (1-2)-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl-(1-4)-β-D-glucopyranosyl (1-6)-β-D-glucopyranosyl ester. This paper discribes in detail the application of 2D NMR techniques in the structure el ucidation of this complex natural product.

Key words: Beesia calthaefolia, Spectroscopic analysis, 2D NMR, Oleanolic triterpenic glycoside

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