波谱学杂志

• 研究论文 •    下一篇

一种脑代谢研究的有效方法——高分辨率磁共振波谱分析

王克强1, 王利民1, 夏薇2, 余亦华2, 顾兴华1, 贾剑国1, 葛均波1   

  1. 1.复旦大学附属中山医院心血管病研究所 基础医学院解剖教研室,上海 200032;
    2.中国科学院上海有机化学研究所,上海 200032
  • 收稿日期:2001-01-08 修回日期:2001-02-05 出版日期:2001-06-05 发布日期:2001-06-05
  • 作者简介:王克强(1943-),男,医学硕士,教授

A POWERFUL METHOD OF STUDYING METABOLISM IN BRAIN——High Resolution Nuclear Magnetic Resonance Spectroscopy

WANG Ke-qiang1, WANG Li-min1, XIA Wei2, YU Yi-hua2, GU Xing-hua1, JIA Jian-guo1, GE Jun-bo1   

  1. 1.The Medical Center of Fudan University (former Shanghai Medical University, Shanghai 200032);
    2.Shanghai Institute of Organis Chemistry, The Chinese Academy of Science, Shanghai 200032
  • Received:2001-01-08 Revised:2001-02-05 Online:2001-06-05 Published:2001-06-05

摘要:

介绍了一种脑代谢研究的有效方法.成年大鼠脑经漏斗法液氮冷冻,制备脑组织高氯酸提取物并冷冻干燥,所得固体溶于D2O后用1H和31P磁共振波谱(MRS)检测.结果表明:脑高氯酸提取物的磁共振波谱有着极好的分辨率,31P MRS可以分辨出ATP、ADP、磷酸肌酸(PCr)、无机磷以及多种磷酯和糖磷;1H MRS可以分辨出乳酸(Lac)、N-乙酰天冬氨酸(NAA)、胆碱(Cho)、肌酸(Cr)、GABA、肌醇(Ino)、琥珀酸(Suc)以及多种氨基酸.各波峰积分后得到各种物质的相对含量,而这些代谢中间产物的相对含量变化可以反应脑内的代谢状况和脑受损伤情况.

关键词:

波谱

Abstract:

In this paper, a howerful method was introduced to study the metabolism in brain. Adult rats were frozen with liquid-N\-2 through a self-command funnel. A
fter perchloric acid (PCA) extracts were prepared and lyophilized, the residual substance were dissolved in D2O and investigated with 1H and 31P nuclear magnetic resonance spectroscopy (MRS). Result:High-resolution nuclear magnetic resonance spectroscopy of brain tissue was achieved. 31P MRS reveals ATP, ADP, phosphocreatine, inorganic orthophosphate and manifold aliphatic phosphates and sugar phosphates; 1H MRS shows many resonance peaks, including lactate, N-acetylaspartate, choline, creatine, GABA, myo-inositol, succinate and manifold amino acids. The peaks were integrated and the relative values might be used to express the metabolism or damnification of brain.

Key words:

中图分类号: