用NMR方法研究三尖杉酯碱诱导HL-60细胞的凋亡

波谱学杂志

用NMR方法研究三尖杉酯碱诱导HL-60细胞的凋亡

吴昌琳¹，钱旻²，黄永仁¹

1. 华东师范大学分析测试中心，国家教委光谱学与波谱学重点实验室，上海 200062； 
2. 华东师范大学生物系，上海 200062

收稿日期:2002-04-23 修回日期:2002-06-24 出版日期:2002-12-05 发布日期:2002-12-05
作者简介:吴昌琳（1965-），女，江西赣州人，博士研究生，讲师，核磁共振波谱专业.
基金资助:
国家自然科学基金资助项目（39870649），上海市科委重点资助项目（00JC14049）.

NMR STUDIES Of HARRINGTONINE INDUCED APOPTOSIS IN HL-60 CELLS

WU Chang-lin¹, QIAN Min², HUANG Yong-ren¹

1. Key Laboratory of Optical and Magnetic Resonance Spectroscopy for Education Ministry, Physics Department of East Chins Normal University, Shanghai 200062, China；
2. Biology Department of East China Normal University，Shanghai 200062, China

Received:2002-04-23 Revised:2002-06-24 Online:2002-12-05 Published:2002-12-05
Supported by:
国家自然科学基金资助项目（39870649），上海市科委重点资助项目（00JC14049）.

摘要/Abstract

摘要：

运用核磁共振波谱（NMR）研究三尖杉酯碱（HT）诱导人早幼粒白血病细胞（HL-60）凋亡中三磷酸腺苷（ATP）、磷酸单脂（PME）及脂类的代谢变化. 经HT作用2 h后，从³¹P NMR中发现HL-60细胞中ATP、PME含量减少；而在¹H NMR中甲基峰有明显的增强，胆碱峰稍微减弱. 这些现象的研究将有利于增进对细胞凋亡发生机制的认识.

关键词: NMR, HL-60, 三尖杉酯碱

Abstract:

NMR spectroscopy methods were used to study the metabolic changes of A TP, PME and lipid during harringtonine (HT) induced apoptosis in HL-60 cell. After HL-60 cells were treated with HT for two hours, it was observed by ³¹P-NMR sp ectroscopy that the levels of ATP and PME decreased significantly and intracellu lar pH increased significantly. It was also found by ¹H-NMR a significa nt increa se in the signal intensity of methylene resonance and a slight decrease in choli ne peak in treated HL-60 cells. The present investigation will be helpful for understanding the mechanism of Harringtonine induced apoptosis in HL-60 cells.

Key words: NMR, spectroscopy, HL-60, harringtonine (HT)

中图分类号:

O482.53

吴昌琳1，钱旻2，黄永仁1. 用NMR方法研究三尖杉酯碱诱导HL-60细胞的凋亡[J]. 波谱学杂志.

WU Chang-lin1, QIAN Min2, HUANG Yong-ren1. NMR STUDIES Of HARRINGTONINE INDUCED APOPTOSIS IN HL-60 CELLS[J]. Chinese Journal of Magnetic Resonance.

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