19F固体核磁共振技术研究膜蛋白相互作用的进展

doi:10.11938/cjmr20182618

摘要/Abstract

摘要： 固体核磁共振技术因其可实现细胞膜环境中的蛋白质结构研究而广受关注.¹⁹F元素由于灵敏度高、天然丰度高，无生物背景等优点，被广泛应用于生物核磁共振技术中.氟标固体核磁共振技术常被用于细胞膜中蛋白质的相互作用研究，如：抗菌肽与细胞膜的相互作用、聚合膜蛋白结构分析等.此篇综述介绍了常用的蛋白质氟标修饰的实验方法，总结了常用的¹⁹F生物固体核磁共振实验技术，以及介绍了应用¹⁹F固体核磁共振研究膜蛋白的成功案例.此外，此篇综述讨论了¹⁹F固体核磁共振技术在蛋白质研究中的局限性.

关键词: ¹⁹F固体核磁共振, 蛋白相互作用, 综述, 膜蛋白

Abstract: The ¹⁹F nucleus has a high gyromagnetic ratio that is comparable to that of proton. Due to its high sensitivity and low background in the biological systems, ¹⁹F labeling has emerged as an attractive approach in biological solid-state NMR studies. In this review, we summarized the widely used ¹⁹F-labeling strategies, ¹⁹F solid-state NMR spectroscopy techniques to probe membrane protein interactions, and the recent applications of ¹⁹F solid-state NMR in characterizing of membrane proteins. The limitations of ¹⁹F solid-state NMR were also highlighted.

Key words: ¹⁹F solid-state NMR, protein interaction, review, membrane protein

中图分类号:

O482.53

徐小俊, 王申林. ¹⁹F固体核磁共振技术研究膜蛋白相互作用的进展[J]. 波谱学杂志, 2019, 36(2): 238-251.

XU Xiao-jun, WANG Shen-lin. Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 238-251.

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¹⁹F固体核磁共振技术研究膜蛋白相互作用的进展

Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR

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