波谱学杂志 ›› 1993, Vol. 10 ›› Issue (3): 267-273.

• 研究论文 • 上一篇    下一篇

23Na NMR研究竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na+通透性的影响

庞素珍, 陈云俊, 乐加昌, 秦静芬, 王海云, 王天盾, 孙继山   

  1. 中国科学院生物物理研究所, 北京 100101
  • 收稿日期:1992-08-27 修回日期:1992-12-22 出版日期:1993-09-05 发布日期:2018-01-20
  • 基金资助:
    国家自然科学基金

THE EFFECT OF HB-PHOTOSENSITIZED DAMAGE ON Na+ PERMEABILITY OF MOUSE ASCITIC HEPATOMA CELLS

Pang Suzhen, Cheng Yunjun, Yue Jiachang, Qing Jingfen, Wang Haiyun, Wang Tiandun, Sun Jishan   

  1. Institute of Biophysics Academia Sinica, Beijing 100101
  • Received:1992-08-27 Revised:1992-12-22 Online:1993-09-05 Published:2018-01-20

摘要: 23Na NMR技术并借助于水溶性位移试剂Dy(PPPi)27-研究了新型光敏剂-竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na+通透性的影响。实验结果表明:在乙素光敏作用下,细胞内Na+浓度随曝光时间的延长和乙素浓度增高而增加。同时观察到细胞外23Naout NMR峰的化学位移(δ值)随光照时间增加而向低场位移。分析表明:化学位移的改变与细胞膜损伤引起细胞内K+泄漏和K+、Na+竞争位移试剂有关。因此,化学位移的改变也可作为光敏损伤的指标。本文还就Na+平衡失调与细胞死亡之间的关系进行了讨论。

关键词: 竹红菌乙素, 腹水肝癌细胞, 23Na NMR, 通透性

Abstract: In this paper 23Na NMR technique with shift reagent Dy(PPPi)27- was used to study a new type of photosensitizer-Hypocrellin B which photosensitized damage on Na+ permeability of ascitic hepatoma cells. The experimental results show that intracellular Na+ concentration increases with both light dose and photosensitizer concentration. It was also observed that the chemical shift of 23Naout NMR peak moved to downfieid as illumination time was increased in the presence of HB.
Analysis indicates that the changes of chemical shift relate to the loss of intraceilular K+ and K+ competes with Na+ for shift reagent. Therefore, the changes of chemical shift may be an index of photosensitized damage. The relation between the increase of Na+ concentration and cell death was discussed.

Key words: Hypocrellin B, Ascitic hepatoma cells, 23Na NMR, Na+ permeability