用23Na NMR研究竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na+通透性的影响

波谱学杂志 ›› 1993, Vol. 10 ›› Issue (3): 267-273.

用²³Na NMR研究竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na⁺通透性的影响

庞素珍, 陈云俊, 乐加昌, 秦静芬, 王海云, 王天盾, 孙继山

中国科学院生物物理研究所, 北京 100101

收稿日期:1992-08-27 修回日期:1992-12-22 出版日期:1993-09-05 发布日期:2018-01-20
基金资助:
国家自然科学基金

THE EFFECT OF HB-PHOTOSENSITIZED DAMAGE ON Na⁺ PERMEABILITY OF MOUSE ASCITIC HEPATOMA CELLS

Pang Suzhen, Cheng Yunjun, Yue Jiachang, Qing Jingfen, Wang Haiyun, Wang Tiandun, Sun Jishan

Institute of Biophysics Academia Sinica, Beijing 100101

Received:1992-08-27 Revised:1992-12-22 Online:1993-09-05 Published:2018-01-20

摘要/Abstract

摘要： 用²³Na NMR技术并借助于水溶性位移试剂Dy(PPPi)₂^7-研究了新型光敏剂-竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na⁺通透性的影响。实验结果表明:在乙素光敏作用下,细胞内Na⁺浓度随曝光时间的延长和乙素浓度增高而增加。同时观察到细胞外²³Na_out NMR峰的化学位移(δ值)随光照时间增加而向低场位移。分析表明:化学位移的改变与细胞膜损伤引起细胞内K⁺泄漏和K⁺、Na⁺竞争位移试剂有关。因此,化学位移的改变也可作为光敏损伤的指标。本文还就Na⁺平衡失调与细胞死亡之间的关系进行了讨论。

关键词: 竹红菌乙素, 腹水肝癌细胞, ²³Na NMR, 通透性

Abstract: In this paper ²³Na NMR technique with shift reagent Dy(PPPi)₂^7- was used to study a new type of photosensitizer-Hypocrellin B which photosensitized damage on Na⁺ permeability of ascitic hepatoma cells. The experimental results show that intracellular Na⁺ concentration increases with both light dose and photosensitizer concentration. It was also observed that the chemical shift of ²³Na_out NMR peak moved to downfieid as illumination time was increased in the presence of HB.
Analysis indicates that the changes of chemical shift relate to the loss of intraceilular K⁺ and K⁺ competes with Na⁺ for shift reagent. Therefore, the changes of chemical shift may be an index of photosensitized damage. The relation between the increase of Na⁺ concentration and cell death was discussed.

Key words: Hypocrellin B, Ascitic hepatoma cells, ²³Na NMR, Na⁺ permeability

庞素珍, 陈云俊, 乐加昌, 秦静芬, 王海云, 王天盾, 孙继山. 用²³Na NMR研究竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na⁺通透性的影响[J]. 波谱学杂志, 1993, 10(3): 267-273.

Pang Suzhen, Cheng Yunjun, Yue Jiachang, Qing Jingfen, Wang Haiyun, Wang Tiandun, Sun Jishan. THE EFFECT OF HB-PHOTOSENSITIZED DAMAGE ON Na⁺ PERMEABILITY OF MOUSE ASCITIC HEPATOMA CELLS[J]. Chinese Journal of Magnetic Resonance, 1993, 10(3): 267-273.

[1]	田建广, 夏照帆, 叶朝辉. 活体²³Na NMR及在生物医学中的应用[J]. 波谱学杂志, 2000, 17(5): 419-425.
[2]	孙健, 刘扬, 陈德文, 张启元, 安静仪. 竹红菌乙素(HB)在C₆₀与三乙胺分子间电子转移反应中的光催化作用[J]. 波谱学杂志, 1999, 16(4): 319-322.
[3]	任吉民, 张善荣, 刘爱琢, 裴奉奎. 稀土离子对人血清白蛋白动态结构性质影响的²³Na NMR研究[J]. 波谱学杂志, 1995, 12(3): 253-259.
[4]	周哲, 丛蓉娟, 黄文秀, 蔡汉龙, 吴钦义, 林东海. 垂直场中稀土离子Dy³⁺对²³Na NMR的影响[J]. 波谱学杂志, 1993, 10(4): 441-446.

用²³Na NMR研究竹红菌乙素光敏损伤对鼠腹水肝癌细胞Na⁺通透性的影响

THE EFFECT OF HB-PHOTOSENSITIZED DAMAGE ON Na⁺ PERMEABILITY OF MOUSE ASCITIC HEPATOMA CELLS

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