波谱学杂志 ›› 1993, Vol. 10 ›› Issue (1): 69-74.

• 研究论文 • 上一篇    下一篇

二维核磁共振谱测定植物新环肽-太子参环肽A和B中氨基酸序列

谭宁华1, 赵守训2, 王德祖2, 张宏杰2, 陈昌祥2, 周俊1   

  1. 1. 中国药科大学植物化学室, 南京 210009;
    2. 中国科学院昆明植物所 植物化学开放实验室, 昆明 650204
  • 收稿日期:1992-06-09 修回日期:1992-08-31 出版日期:1993-03-05 发布日期:2018-01-20
  • 基金资助:
    中国科学院昆明植物所植物化学开放实验室资助项目

DETERMINATION OF AMINO ACID SEQUENCES OF TWO NEW CYCLOPEPTIDES BY 2D NMR

Tan Ninghua1, Wang Dezu2, Zhang Hongjie2, Chen Changxiang2, Zhou Jun2, Zhao Shouxun1   

  1. 1. Department of Phyrochemistr China Pharmaceutical University, Nanjing, 210009;
    2. Laboratory of Phytochcmistry, Kunming Institute of Botany, Academia Sinica, Kunming, 650204
  • Received:1992-06-09 Revised:1992-08-31 Online:1993-03-05 Published:2018-01-20

摘要: 在植物新环肽[太子参环肽A(HA)和B(HB)]的结构研究中,应用COLOC谱较成功地解决了其氨基酸序列。结果表明当J值选择在6Hz-15Hz时,COLOC谱能满意地给出氮上含有活泼氢的氨基酸序列。

关键词: 二维核磁共振, COLOC, 氨基酸序列测定, 太子参环肽

Abstract: By combination of several NMR techniques (including 1H-1H、1H-13C COSY、RELAY, and COLOC), the spin systems and sequences of amino acid residues of heterophyllin A and B, two new cyclopeptides which were isolated from Pseudostellaria heterophylla, have been successfully elucidated. The results indicated the sequences of amino acids with NH can be satisfactorily determined by COLOC when the nJ(XH) were 6Hz to 15Hz.

Key words: 2D NMR, COLOC, Determination of sequences of amino acids, Cyclopeptides, Heterophyllin A, Heterophyllin B